哺乳期奶牛妊娠丢失诱导前后血清妊娠相关糖蛋白、特异性蛋白 B 和孕酮浓度的变化。

Changes in serum pregnancy-associated glycoprotein, pregnancy-specific protein B, and progesterone concentrations before and after induction of pregnancy loss in lactating dairy cows.

机构信息

Department of Dairy Science, University of Wisconsin, Madison 53706, USA.

出版信息

J Dairy Sci. 2012 Feb;95(2):683-97. doi: 10.3168/jds.2011-4609.

DOI:10.3168/jds.2011-4609

Abstract

Lactating crossbred dairy cows were synchronized to receive a timed artificial insemination (TAI), and blood samples were collected from all cows from TAI until pregnancy diagnosis 39 d after TAI (period 1), and from pregnant cows from onset of treatment until the end of the experiment (period 2). Cows diagnosed pregnant 39 d after TAI were randomly assigned to 1 of 3 treatments to receive (1) an i.m. injection of saline (CON, n=10); (2) an i.m. injection of PGF(2α) (PGF, n=10); or (3) an intrauterine infusion of 120 mL of hypertonic saline (INF, n=9). During period 1, serum pregnancy-associated glycoprotein (PAG) concentrations began to increase in pregnant cows by 25 d after TAI and differed from those in nonpregnant cows by 27 d after TAI, whereas serum pregnancy-specific protein B (PSPB) concentrations in pregnant cows differed from those in nonpregnant cows by 22 d after TAI. During period 2, time from treatment to cessation of the embryonic heartbeat was greater for PGF than for INF cows (36.0±5.7 vs. 0.2±0.1 h, respectively), and time from treatment to conceptus disappearance was greater for INF than for PGF cows (7.1±3.3 vs. 1.9±0.3 d, respectively). Overall, progesterone concentration was greater for CON and INF than for PGF cows (8.7±2.8, 8.2±3.1, and 1.0±2.3 ng/mL, respectively) due to luteal regression for PGF cows and corpus luteum maintenance for CON and INF cows. Serum PAG and PSPB concentrations differed among CON cows and PGF and INF cows beginning 1 and 2.5 d after treatment for PAG and PSPB, respectively. By 9.5 d after treatment, PAG and PSPB concentrations were similar to those of nonpregnant cows. We conclude that although timing of conceptus expulsion occurred 5.2 d later for INF than for PGF cows, serum PAG and PSPB concentrations decreased at a similar rate from the onset of treatment for both models of pregnancy loss evaluated.

摘要

哺乳期杂交奶牛被同步进行定时人工授精（TAI），从 TAI 开始直到妊娠诊断后 39 天（第 1 期），所有奶牛均采集血液样本，从妊娠奶牛开始治疗到实验结束（第 2 期）。TAI 后 39 天诊断为妊娠的奶牛被随机分配到 3 种处理方法中的 1 种，分别接受（1）肌肉注射生理盐水（CON，n=10）；（2）肌肉注射 PGF（2α）（PGF，n=10）；或（3）宫内输注 120mL 高渗盐水（INF，n=9）。在第 1 期，妊娠相关糖蛋白（PAG）浓度在 TAI 后 25 天开始在妊娠奶牛中升高，并在 TAI 后 27 天与非妊娠奶牛有所区别，而在妊娠奶牛中，妊娠特异性蛋白 B（PSPB）浓度在 TAI 后 22 天与非妊娠奶牛有所区别。在第 2 期，PGF 组奶牛从治疗到胚胎心跳停止的时间长于 INF 组（36.0±5.7 比 0.2±0.1 h），而 INF 组奶牛从治疗到胚胎消失的时间长于 PGF 组（7.1±3.3 比 1.9±0.3 d）。总的来说，由于 PGF 组奶牛黄体退化，CON 和 INF 组奶牛黄体维持，PGF、CON 和 INF 组奶牛的孕酮浓度分别为 8.7±2.8、8.2±3.1 和 1.0±2.3ng/mL。PGF 和 INF 组奶牛从治疗开始后 1 和 2.5 天，血清 PAG 和 PSPB 浓度与 CON 奶牛和 PGF 奶牛之间存在差异。治疗后 9.5 天，PAG 和 PSPB 浓度与非妊娠奶牛相似。我们的结论是，尽管 INF 组奶牛胚胎排出时间比 PGF 组晚 5.2 天，但从两种妊娠丢失模型的治疗开始，血清 PAG 和 PSPB 浓度的下降速度相似。