Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
Anal Bioanal Chem. 2012 Mar;402(7):2385-93. doi: 10.1007/s00216-012-5736-x. Epub 2012 Jan 28.
Thermoresponsive microgels have been shown to be an excellent platform for designing sensor materials. Recently, poly (N-isopropylacrylamide)-co-acrylic acid (pNIPAm-co-AAc) microgel based etalon materials have been described as direct sensing materials that can be designed to have a single, unique color. These color tunable materials show immense promise for sensing due to their spectral sensitivity and bright visual color. Here, we describe a proof-of-concept for etalon sensing of glucose. We found that aminophenylboronic acid (APBA)-functionalized pNIPAm-co-AAc microgels in an etalon respond to 3 mg/mL glucose concentrations by red shifting their reflectance peaks by 110 nm up to 150 nm. Additionally, APBA-functionalized pNIPAm-co-AAc microgels have a depressed volume phase transition temperature at 18-20 °C, which shifts to 24-26 °C after glucose binding. We also demonstrate that these materials show a marked visual color change, which is a first step towards developing direct read-out sensor devices.
温敏微凝胶已被证明是设计传感器材料的绝佳平台。最近，聚（N-异丙基丙烯酰胺）-共-丙烯酸（pNIPAm-co-AAc）微凝胶作为标准材料已被描述为直接传感材料，可以设计为具有单一、独特的颜色。由于这些颜色可调材料具有光谱敏感性和鲜艳的视觉颜色，因此在传感方面具有巨大的应用潜力。在这里，我们描述了一种基于标准的葡萄糖感应的概念验证。我们发现，在标准中，氨苯基硼酸（APBA）功能化的 pNIPAm-co-AAc 微凝胶对 3mg/mL 葡萄糖浓度的响应是通过将其反射峰红移 110nm 至 150nm。此外，APBA 功能化的 pNIPAm-co-AAc 微凝胶的体积相转变温度在 18-20°C 时降低，在与葡萄糖结合后转变为 24-26°C。我们还证明，这些材料表现出明显的视觉颜色变化，这是开发直接读出传感器设备的第一步。
