《黑曲霉对美洛昔康的生物转化：碳源和氮源的意义》
Biotransformation of Meloxicam by Cunninghamella blakesleeana: Significance of Carbon and Nitrogen Source.
机构信息
Department of Microbiology, Kakatiya University, H. No. 16-4-168, Fort Road, Warangal, 506002 Andhra Pradesh India.
出版信息
Indian J Microbiol. 2011 Jan;51(1):82-7. doi: 10.1007/s12088-011-0099-0. Epub 2011 Jan 30.
Influence of carbon and nitrogen source, on biotransformation of meloxicam was studied by employing Cunninghamella blakesleeana NCIM 687 with an aim to achieve maximum transformation of meloxicam and in search of new metabolites. The transformation was confirmed by HPLC and based on LC-MS-MS data and previous reports the metabolites were predicted as 5-hydroxymethyl meloxicam, 5-carboxy meloxicam and a novel metabolite. The quantification of metabolites was performed using HPLC peak areas. The results obtained indicate that glucose as carbon source, ammonium nitrate as nitrogen source, were found to be optimum for maximum transformation of meloxicam. The study suggests the significance of these factors in biotransformation of meloxicam using microbial cultures. The fermentation was scaled up to 1 l level.
采用毕赤酵母（Cunninghamella blakesleeana NCIM 687）研究了碳源和氮源对美洛昔康生物转化的影响，目的是实现美洛昔康的最大转化，并寻找新的代谢产物。通过 HPLC 确认了转化，并根据 LC-MS-MS 数据和以往的报告，预测代谢产物为 5-羟甲基美洛昔康、5-羧基美洛昔康和一种新型代谢产物。使用 HPLC 峰面积对代谢产物进行定量。结果表明，葡萄糖作为碳源，硝酸铵作为氮源，有利于美洛昔康的最大转化。该研究表明了这些因素在微生物培养物中美洛昔康生物转化中的重要性。发酵扩大到 1 L 规模。
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