化学物质诱导克隆鼠 M2 成纤维细胞产生哇巴因抗性和恶性转化的突变。

Induction of mutation to ouabain resistance and of malignant transformation by chemicals in cloned mouse M2 fibroblasts.

机构信息

Department of Toxicology, University of Hamburg Medical School, Martinistrasse 52, 2000 Hamburg 20, GFR.

出版信息

Carcinogenesis. 1980 Mar;1(3):215-8. doi: 10.1093/carcin/1.3.215.

DOI:10.1093/carcin/1.3.215

Abstract

Malignant transformation and mutation to ouabain-resistance is induced in cloned M2 mouse fibroblasts in vitro by treatment with N-methyl-N'-nitro-N-nitrosoguanidine and two derivatives of polycyclic aromatic hydrocarbons, the 3,4-diol derived from 7-methylbenz [a]anthracene and the 7,8-diol-9,10-oxide derived from benzo[a]pyrene. Transformation is an event more frequent than mutation, the ratio of transformation to mutation frequencies varied between 24 and 1118. Another hydrocarbon derivative, the 9,10-diol-7,8-oxide derived from benzo[a]pyrene, did induce ouabain-resistance without causing transformation. Induced ouabain-resistant clones did not behave like transformants, and transformants did not exhibit ouabain resistance. The significance of these findings is discussed.

摘要

在体外，用 N-甲基-N'-硝基-N-亚硝基胍和两种多环芳烃衍生物（7-甲基苯并[a]蒽衍生的 3,4-二醇和苯并[a]芘衍生的 7,8-二醇-9,10-氧化物）处理克隆的 M2 小鼠成纤维细胞，可诱导恶性转化和突变，产生对哇巴因的抗性。转化是比突变更频繁发生的事件，转化与突变频率的比值在 24 到 1118 之间变化。另一种烃类衍生物，苯并[a]芘衍生的 9,10-二醇-7,8-氧化物，在不引起转化的情况下也能诱导哇巴因抗性。诱导产生的哇巴因抗性克隆并不表现出转化细胞的特征，而转化细胞也不表现出哇巴因抗性。这些发现的意义将被讨论。