化学致癌物可使可转化的C3H/10T1/2 Cl 8小鼠成纤维细胞发生突变，从而产生对哇巴因的抗性。
Chemical carcinogens produce mutations to ouabain resistance in transformable C3H/10T1/2 Cl 8 mouse fibroblasts.
作者信息
Landolph J R, Heidelberger C
出版信息
Proc Natl Acad Sci U S A. 1979 Feb;76(2):930-4. doi: 10.1073/pnas.76.2.930.
Abstract
Chemical carcinogens induce mutations to ouabain resistance in the transformable mouse fibroblast cell line C3H/10T1/2 Cl 8. The mutant phenotype is stable and heritable in the absence of selective agent, and dose--response curves for mutant frequency were obtained with N-menthyl-N'-nitro-N-nitrosoguanidine, 3-methylcholanthrene, benzo[a]-pyrene, N-acetoxy-N-2-acetylaminofluorene, and the anti-7,8-diol-9,10-epoxide of benzo[a]pyrene. The ratio of the malignant transformation frequency to the mutation frequency was 12 for benzo[a]pyrene and 21 for N-acetoxy-N-2-acetylaminofluorene. The development of the mutational assay reported here allows the use of this permanent cell line for comparison of mutation and transfomration frequencies and as a screening system for xenobiotics that pose mutagenic or carcinogenic hazards to mammalian cells.
摘要
化学致癌物可诱导可转化的小鼠成纤维细胞系C3H/10T1/2 Cl 8发生对哇巴因耐药的突变。在没有选择剂的情况下，突变表型是稳定且可遗传的，并且用N-甲基-N'-硝基-N-亚硝基胍、3-甲基胆蒽、苯并[a]芘、N-乙酰氧基-N-2-乙酰氨基芴以及苯并[a]芘的反式-7,8-二醇-9,10-环氧化物获得了突变频率的剂量-反应曲线。苯并[a]芘的恶性转化频率与突变频率之比为12，N-乙酰氧基-N-2-乙酰氨基芴为21。本文报道的突变检测方法的开发使得该永久细胞系可用于比较突变频率和转化频率，并作为对哺乳动物细胞具有诱变或致癌危害的外源化学物的筛选系统。
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