Department of Analytical Chemistry, Chemical Faculty, Gdańsk University of Technology (GUT), Gdańsk, Poland.
Anal Chim Acta. 2012 Feb 24;716:24-7. doi: 10.1016/j.aca.2011.02.059. Epub 2011 Mar 5.
In the present study, an analytical procedure was developed for the determination of short-chain fatty acids (SCFAs) in landfill leachate and municipal wastewater employing injection of aqueous samples to gas chromatograph with flame ionization detector (GC-FID). Chromatographic conditions such as a separation system, injection volume, oven temperature program were investigated and selected. With two columns, one with a polar (polyethylene glycol) and one with a non-polar (dimethylpolisiloxane) stationary phase, good separation of SCFAs, containing from 2 to 8 carbon atoms, was achieved. The sample volume was 2 μL and the temperature program 80°C (30 s) then 7 °C min(-1) to 220 °C (2 min). LOQs values were below 0.25 mg L(-1). The concentrations of the acids in the landfill leachate studied ranged from 0.45±0,059 (average ± extended uncertainty) mg L(-1) for pentanoic acid to 15.2±0.73 mg L(-1) for ethanoic acid. Concentrations of SCFAs in the municipal wastewater were lower than LOQs.
在本研究中，采用注射水样进样到带有火焰离子化检测器的气相色谱仪（GC-FID）的方法，开发了一种用于测定垃圾渗滤液和城市废水中短链脂肪酸（SCFAs）的分析程序。对色谱条件，如分离系统、进样量、柱温程序等进行了考察和选择。使用两根柱，一根具有极性（聚乙二醇），一根具有非极性（二甲基聚硅氧烷）固定相，实现了含有 2 至 8 个碳原子的 SCFAs 的良好分离。样品体积为 2 μL，程序升温为 80°C（30 s），然后以 7°C min(-1)升温至 220°C（2 min）。LOQs 值低于 0.25 mg L(-1)。所研究的垃圾渗滤液中酸的浓度范围为从戊酸的 0.45±0.059（平均值±扩展不确定度）mg L(-1)到乙酸的 15.2±0.73 mg L(-1)。城市废水中 SCFAs 的浓度低于 LOQs。
