Deng Guozhe, Xie Li, Xu Shengjia, Kang Xuejun, Ma Jizheng
Key Laboratory of Child Development and Learning Science of Ministry of Education of China, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
College of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing 210038, China.
Polymers (Basel). 2021 Nov 11;13(22):3906. doi: 10.3390/polym13223906.
Short-chain fatty acids (SCFAs) are among the active metabolites in biological process both in the intestinal tract and the bioconversion of organic wastes, which has resulted in various human diseases and environmental problems. In order to accurately detect SCFAs, we introduced a novel extraction sorbent. Electrospun polyacrylonitrile (PAN) nanofiber membrane was synthesized, then poly (3, 4-Ethylenedioxythiophene) (PEDOT) was deposited onto the surface of electrospun PAN nanofibers by in situ polymerization. The morphology of the composite PAN/PEDOT nanofiber was characterized by scanning electronic microscopy (SEM) and FTIR spectrum. PAN/PEDOT was used to isolate and concentrate the SCFAs in waste water and fecal samples before gas chromatography mass spectrometry (GC-MS) analysis. The analytical method was evaluated systematically, and low limits of detection (LODs) of 0.34-0.87 μg/L and good linearity (R ≥ 0.9953) were obtained. The method was applied successfully for the determination of SCFAs in waste water and fecal samples, with good recovery (87.5-104.6%) and satisfactory reproducibility (relative standard deviation: 6.5-14.1%). The results indicated that the proposed method can be used as a potential approach for the determination of SCFAs with high sensitivity in waste water and biological samples.
短链脂肪酸（SCFAs）是肠道生物过程以及有机废物生物转化过程中的活性代谢产物之一，这导致了各种人类疾病和环境问题。为了准确检测SCFAs，我们引入了一种新型萃取吸附剂。合成了静电纺聚丙烯腈（PAN）纳米纤维膜，然后通过原位聚合将聚（3,4-乙撑二氧噻吩）（PEDOT）沉积到静电纺PAN纳米纤维表面。通过扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）对复合PAN/PEDOT纳米纤维的形态进行了表征。在气相色谱-质谱联用（GC-MS）分析之前，使用PAN/PEDOT对废水和粪便样品中的SCFAs进行分离和富集。对该分析方法进行了系统评估，获得了0.34 - 0.87 μg/L的低检测限（LODs）和良好的线性关系（R≥0.9953）。该方法成功应用于废水和粪便样品中SCFAs的测定，回收率良好（87.5 - 104.6%），重现性令人满意（相对标准偏差：6.5 - 14.1%）。结果表明，所提出的方法可作为一种潜在的方法，用于高灵敏度地测定废水和生物样品中的SCFAs。
