UMR 1272, UPMC-INRA, Physiologie de l'Insecte: Signalisation et Communication, Université Paris VI, Bâtiment A, 7 quai Saint Bernard, 75005 Paris, France.
Gen Comp Endocrinol. 2012 Apr 1;176(2):158-66. doi: 10.1016/j.ygcen.2012.01.005. Epub 2012 Jan 20.
In the male moth, Agrotis ipsilon, the behavioral response and neuronal sensitivity in the primary olfactory center, the antennal lobe (AL), to sex pheromone increase with age and juvenile hormone (JH) biosynthesis. Although JH has been shown to control this age-dependent plasticity, the underlying signaling pathway remains obscure. In this context, we cloned a full cDNA encoding the Krüppel homolog 1 transcription factor (AipsKr-h1) of A. ipsilon, which was found to be predominantly expressed in ALs, where its amount increased concomitantly with age and sex pheromone responses. Conversely, the expression of AipsKr-h1 protein in the antenna was age-independent. Moreover, the administration of JH in immature males or fluvastatin, an inhibitor of JH biosynthesis, in mature males induced an increase or a decline of the AipsKr-h1 protein level in ALs, respectively. This effect was suppressed with a combined injection of fluvastatin and JH. Our results showed that Aipskr-h1 is a JH-upregulated gene that might mediate JH action on central pheromone processing, modulating sexual behavior in A. ipsilon.
在雄性斜纹夜蛾（Agrotis ipsilon）中，其对性信息素的行为反应和初级嗅觉中枢（触角叶，antennal lobe，AL）的神经元敏感性会随着年龄和保幼激素（juvenile hormone，JH）生物合成的增加而增强。尽管已经证明 JH 可以控制这种年龄依赖性的可塑性，但潜在的信号通路仍然不清楚。在这种情况下，我们克隆了斜纹夜蛾 Krüppel 同源物 1 转录因子（AipsKr-h1）的全长 cDNA，该基因主要在 AL 中表达，其含量随着年龄和性信息素反应的增加而增加。相反，AipsKr-h1 蛋白在触角中的表达与年龄无关。此外，在未成熟雄性个体中注射 JH 或在成熟雄性个体中注射 JH 生物合成抑制剂 fluvastatin，分别会导致 AL 中 AipsKr-h1 蛋白水平的增加或下降。联合注射 fluvastatin 和 JH 可以抑制这种效应。我们的研究结果表明，Aipskr-h1 是一种 JH 上调基因，可能介导 JH 对中央性信息素处理的作用，从而调节斜纹夜蛾的性行为。
