IPICyT, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José No. 2055, Lomas 4ª sección, 78216 San Luis Potosí, SLP, Mexico.
J Proteomics. 2012 Apr 3;75(7):2160-9. doi: 10.1016/j.jprot.2012.01.015. Epub 2012 Jan 25.
Papaya (Carica papaya L.) is a climacteric fruit susceptible to postharvest losses due to the ethylene-induced ripening. The inhibitor of ethylene action, 1-methylcyclopropene (1-MCP), has been used worldwide as a safe postharvest non-toxic agent, but the physiological and biochemical modifications induced by 1-MCP are not well understood. Using the 2-DE analysis, we report the changes in the protein profiles after 6 and 18 days of postharvest and the effect of the effect of 1-MCP treatment on fruits. Twenty seven protein spots showing differences in abundance during ripening were successfully identified by nano-LC-ESI/MS/MS. Some spots corresponded to the cell wall degrading enzymes related to fruit ripening; others were involved in oxidative damage protection, protein folding, and cell growth and survival that were induced by 1-MCP. This is the first proteomic report analyzing the effect of 1-MCP in papaya ripening. The present data will help to shed light on papaya fruit ripening process.
番木瓜（Carica papaya L.）是一种呼吸跃变型果实，容易在采后损失，这是由于乙烯诱导的成熟作用。乙烯作用抑制剂 1-甲基环丙烯（1-MCP）已被全世界用作安全无毒的采后处理剂，但 1-MCP 诱导的生理生化变化尚不清楚。本研究通过 2-DE 分析，报告了采后 6 天和 18 天后蛋白质图谱的变化，以及 1-MCP 处理对果实的影响。通过纳升液相色谱-电喷雾-串联质谱（nano-LC-ESI/MS/MS）成功鉴定了 27 个在成熟过程中丰度发生变化的蛋白点。一些蛋白点与与果实成熟有关的细胞壁降解酶有关；另一些则与氧化损伤保护、蛋白质折叠、细胞生长和存活有关，这些都是 1-MCP 诱导的。这是首次对 1-MCP 处理番木瓜成熟过程的蛋白质组学研究进行分析。本研究数据将有助于揭示番木瓜果实成熟过程。
