College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Comp Immunol Microbiol Infect Dis. 2012 Mar;35(2):197-207. doi: 10.1016/j.cimid.2012.01.006. Epub 2012 Jan 28.
In the current study, four novel avian β-defensins (AvBDs) (AvBD2, 5, 9, and 10) were identified in tissues from the Chinese goose (Anser cygnoides). The antibacterial activity of the AvBDs showed that all of these AvBDs exhibited antibacterial activity against most of the bacteria investigated (P<0.01). In addition, antibacterial activity of all of the AvBDs against Staphylococcus aureus and Pasteurella multocida decreased significantly or was completely abolished at 150mM NaCl (P<0.01). None of the AvBDs showed hemolytic activity. AvBD2 and AvBD10 were expressed widely, whereas AvBD5 and AvBD9 mRNAs were expressed in a limited number of geese tissues. AvBD9 was significantly induced in some immune tissues from geese after Salmonella enteritidis infection. The others were significantly upregulated in small intestine and some immune tissues of the geese (P<0.01). The present results suggest that the AvBDs are part of the host defense mechanism of the goose.
在本研究中，从中国鹅（Anser cygnoides）组织中鉴定出了四种新型禽β-防御素（AvBD）（AvBD2、5、9 和 10）。AvBD 的抗菌活性表明，所有这些 AvBD 对大多数研究的细菌均具有抗菌活性（P<0.01）。此外，所有 AvBD 对金黄色葡萄球菌和多杀巴斯德氏菌的抗菌活性在 150mM NaCl 下显著降低或完全丧失（P<0.01）。AvBD 没有表现出溶血活性。AvBD2 和 AvBD10 广泛表达，而 AvBD5 和 AvBD9 mRNA 仅在少数鹅组织中表达。在感染肠炎沙门氏菌后，AvBD9 在一些鹅的免疫组织中明显被诱导。其他 AvBD 在鹅的小肠和一些免疫组织中被显著上调（P<0.01）。这些结果表明，AvBD 是鹅宿主防御机制的一部分。
