College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China.
Mol Immunol. 2013 Dec;56(4):521-9. doi: 10.1016/j.molimm.2013.05.227. Epub 2013 Aug 1.
Here, we report the characterization of three avian β-defensins (AvBDs) from the goose, named anser_AvBD1, AvBD3, and AvBD6, respectively. All of anser_AvBDs exhibited broad antibacterial activity. In addition, the antibacterial activity of all of the AvBDs against Staphylococcus aureus and Proteus mirabilis decreased significantly in the presence of 100mM NaCl (P<0.01). None of the AvBDs showed hemolytic activity. In order to assess the significance of these anser_AvBDs in the infection of Salmonella enteritidis, mRNA expression of Toll-like receptor (TLR) 4 and anser_AvBDs in tissues of both control and infected geese was evaluated. We observed a significant up-regulation of TLR4, anser_AvBD1, 3, and 6 in some immune tissues evaluated, in response to S. enteritidis infection. These data demonstrated that TLR4 may serve a possible role in eliciting host immune responses to pathogens, and β-defensins may play a pivotal role in the host defense mechanisms of the goose.
在这里，我们分别报道了来自鹅的三种禽β-防御素（AvBD）的特征，分别命名为anser_AvBD1、AvBD3 和 AvBD6。所有的 anser_AvBD 都表现出广泛的抗菌活性。此外，在存在 100mM NaCl 的情况下，所有 AvBD 对金黄色葡萄球菌和奇异变形杆菌的抗菌活性显著降低（P<0.01）。AvBD 均无溶血活性。为了评估这些 anser_AvBD 在肠炎沙门氏菌感染中的意义，我们评估了对照组和感染组鹅组织中 Toll 样受体（TLR）4 和 anser_AvBD 的 mRNA 表达。我们观察到，在一些免疫组织中，TLR4、anser_AvBD1、3 和 6 的表达显著上调，以响应肠炎沙门氏菌感染。这些数据表明，TLR4 可能在引发宿主对病原体的免疫反应中起重要作用，而β-防御素可能在鹅的宿主防御机制中发挥关键作用。
