The Food and Environment Research Agency, Sand Hutton, York YO41 1LZ, UK.
Peptides. 2012 Mar;34(1):44-50. doi: 10.1016/j.peptides.2012.01.009. Epub 2012 Jan 20.
The adipokinetic hormones (AKHs) from the corpora cardiaca (CC) of representative species from all three subfamilies of the Sphingidae (hawkmoths) were investigated using matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) and liquid chromatography electrospray ion trap mass spectrometry (LC-ESI MS), including a re-examination of the AKH complement of the tobacco hawkmoth, Manduca sexta. In addition to larvae and adults of M. sexta (subfamily: Sphinginae), adults from the following subfamilies were examined: Macroglossinae (large elephant hawkmoth, Deilephila elpenor), Smerinthinae (poplar hawkmoth, Laothoe populi and eyed hawkmoth, Smerinthus ocellata), and Sphinginae (death's head hawkmoth, Acherontia atropos). All moths are shown to have the nonapeptide Manse-AKH (pELTFTSSWGamide) [corrected] in their CC, together with a second AKH, which, on the basis of mass ions (M+Na, M+K) and partial sequence analysis is identical in all species examined. The structure of this AKH was extracted from the CC [corrected] of adult M. sexta and shown, by ESI-collision-induced dissociation (CID) tandem mass spectrometry (MS/MS), to be a novel decapeptide AKH with a sequence of pELTFSSWGQamide. [corrected]. The new peptide has been code named Manse-AKH-II. Sequence confirmation was obtained from identical MS studies with synthetic Manse-AKH-II and with the native peptide. Manse-AKH-II has significant lipid-mobilizing activity when injected at low dose (5pmol) into newly emerged adult M. sexta. The potential implications of a second AKH, in M. sexta in particular, are discussed in relation to putative receptor(s).
使用基质辅助激光解吸电离飞行时间（MALDI-TOF）和液相色谱电喷雾离子阱质谱（LC-ESI MS）研究了来自鳞翅目三个亚科（天蛾）所有代表物种的心冠腺（CC）中的脂肪激活激素（AKH），包括对烟草天蛾（Manduca sexta）AKH 成分的重新检查。除了 M. sexta（鳞翅目：Sphinginae）的幼虫和成虫外，还检查了以下亚科的成虫：Macroglossinae（大型象鼻虫，Deilephila elpenor）、Smerinthinae（杨扇舟蛾，Laothoe populi 和眼天蛾，Smerinthus ocellata）和 Sphinginae（死神天蛾，Acherontia atropos）。所有飞蛾都在心冠腺中显示出非肽 Manse-AKH（pELTFTSSWGamide）[校正]，以及第二种 AKH，根据质量离子（[M+Na]+，[M+K]+）和部分序列分析，在所有检查的物种中都是相同的。这种 AKH 的结构从成年 M. sexta 的 CC 中提取出来，并通过 ESI 碰撞诱导解离（CID）串联质谱（MS/MS）显示，它是一种具有 pELTFSSWGQamide 序列的新型十肽 AKH。[校正]。该新肽已被命名为 Manse-AKH-II。通过对合成的 Manse-AKH-II 和天然肽进行相同的 MS 研究，获得了序列确认。当以低剂量（5pmol）注入新出现的成年 M. sexta 时，Manse-AKH-II 具有显著的脂质动员活性。特别是在 M. sexta 中，第二种 AKH 的潜在影响，特别是在 M. sexta 中，与假定的受体（s）有关。
