Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, 530004, China.
Sci Rep. 2022 Jun 14;12(1):9892. doi: 10.1038/s41598-022-13590-7.
Neuropeptides and neuropeptide receptors are crucial regulators to insect physiological processes. The 21.0 Gb bases were obtained from Illumina sequencing of two libraries representing the female and male heads of Phauda flammans (Walker) (Lepidoptera: Phaudidae), which is a diurnal defoliator of ficus plants and usually outbreaks in the south and south-east Asia, to identify differentially expressed genes, neuropeptides and neuropeptide receptor whose tissue expressions were also evaluated. In total, 99,386 unigenes were obtained, in which 156 up-regulated and 61 down-regulated genes were detected. Fifteen neuropeptides (i.e., F1b, Ast, NP1, IMF, Y, BbA1, CAP2b, NPLP1, SIF, CCH2, NP28, NP3, PDP3, ARF2 and SNPF) and 66 neuropeptide receptor genes (e.g., A2-1, FRL2, A32-1, A32-2, FRL3, etc.) were identified and well-clustered with other lepidopteron. This is the first sequencing, identification neuropeptides and neuropeptide receptor genes from P. flammans which provides valuable information regarding the molecular basis of P. flammans.
神经肽和神经肽受体是昆虫生理过程的重要调节剂。从代表 Phauda flammans（Walker）（鳞翅目：Phaudidae）雌雄头部的两个文库的 Illumina 测序中获得了 21.0 Gb 碱基，以鉴定差异表达基因、神经肽和神经肽受体，其组织表达也进行了评估。总共获得了 99386 个基因，其中检测到 156 个上调基因和 61 个下调基因。鉴定并聚类了 15 种神经肽（即 F1b、Ast、NP1、IMF、Y、BbA1、CAP2b、NPLP1、SIF、CCH2、NP28、NP3、PDP3、ARF2 和 SNPF）和 66 种神经肽受体基因（如 A2-1、FRL2、A32-1、A32-2、FRL3 等）。这是首次从 P. flammans 中测序、鉴定神经肽和神经肽受体基因，为 P. flammans 的分子基础提供了有价值的信息。
