Departamento de Biotecnologia, DEBIQ, Escola de Engenharia de Lorena, EEL, USP Universidade de São Paulo, P.O. Box 116, 12600-970 Lorena, SP, Brazil.
Bioresour Technol. 2012 Mar;108:134-9. doi: 10.1016/j.biortech.2011.09.026. Epub 2011 Dec 27.
Response surface methodology (RSM), based on a 2(2) full factorial design, evaluated the moisture effects in recovering xylose by diethyloxalate (DEO) hydrolysis. Experiments were carried out in laboratory reactors (10 mL glass ampoules) containing corn stover (0.5 g) properly ground. The ampoules were kept at 160 °C for 90 min. Both DEO concentration and corn stover moisture content were statistically significant at 99% confidence level. The maximum xylose recovery by the response surface methodology was achieved employing both DEO concentration and corn stover moisture at near their highest levels area. We amplified this area by using an overlay plot as a graphical optimization using a response of xylose recovery more than 80%. The mathematical statistical model was validated by testing a specific condition in the satisfied overlay plot area. Experimentally, a maximum xylose recovery (81.2%) was achieved by using initial corn stover moisture of 60% and a DEO concentration of 4% w/w. The mathematical statistical model showed that xylose recovery increases during DEO corn stover acid hydrolysis as the corn stover moisture level increases. This observation could be important during the harvesting of corn before it is fully dried in the field. The corn stover moisture was an important variable to improve xylose recovery by DEO acid hydrolysis.
响应面法（RSM）基于 2(2)完全析因设计，评估了二乙氧草酸酯（DEO）水解回收木糖过程中的水分效应。实验在实验室反应器（10 毫升玻璃安瓿）中进行，其中含有经过适当粉碎的玉米秸秆（0.5 克）。安瓿在 160°C 下保持 90 分钟。DEO 浓度和玉米秸秆水分含量在 99%置信水平下均具有统计学意义。通过在接近最高水平区域同时使用 DEO 浓度和玉米秸秆水分，采用响应面法实现了最大木糖回收率。我们通过使用覆盖图作为图形优化来放大该区域，响应的木糖回收率超过 80%。通过在满足覆盖图区域的特定条件下进行测试，验证了数学统计模型。实验中，通过使用初始玉米秸秆水分 60%和 DEO 浓度 4%（w/w），实现了最大木糖回收率（81.2%）。数学统计模型表明，随着玉米秸秆水分水平的增加，DEO 玉米秸秆酸水解过程中的木糖回收率增加。这一观察结果在玉米完全干燥之前在田间收获时可能非常重要。玉米秸秆水分是通过 DEO 酸水解提高木糖回收率的重要变量。
