Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture(1), 1815 N. University Street, Peoria, IL 61604, USA.
Bioresour Technol. 2013 Feb;130:603-12. doi: 10.1016/j.biortech.2012.12.104. Epub 2012 Dec 22.
Dilute H(3)PO(4) (0.0-2.0%, v/v) was used to pretreat corn stover (10%, w/w) for conversion to ethanol. Pretreatment conditions were optimized for temperature, acid loading, and time using central composite design. Optimal pretreatment conditions were chosen to promote sugar yields following enzymatic digestion while minimizing formation of furans, which are potent inhibitors of fermentation. The maximum glucose yield (85%) was obtained after enzymatic hydrolysis of corn stover pretreated with 0.5% (v/v) acid at 180°C for 15min while highest yield for xylose (91.4%) was observed from corn stover pretreated with 1% (v/v) acid at 160°C for 10min. About 26.4±0.1g ethanol was produced per L by recombinant Escherichia coli strain FBR5 from 55.1±1.0g sugars generated from enzymatically hydrolyzed corn stover (10%, w/w) pretreated under a balanced optimized condition (161.81°C, 0.78% acid, 9.78min) where only 0.4±0.0g furfural and 0.1±0.0 hydroxylmethyl furfural were produced.
用 0.0-2.0%（v/v）的稀 H3PO4预处理玉米秸秆（10%，w/w），以将其转化为乙醇。采用中心复合设计优化了温度、酸负荷和时间的预处理条件。选择最佳的预处理条件，以促进酶解后的糖产量，同时将糠醛等发酵抑制剂的形成降至最低。在 180°C 下用 0.5%（v/v）酸预处理 15min 后，玉米秸秆的最大葡萄糖得率（85%）；在 160°C 下用 1%（v/v）酸预处理 10min 后，玉米秸秆的最大木糖得率（91.4%）。在平衡优化条件（161.81°C、0.78%酸、9.78min）下，用重组大肠杆菌 FBR5 从酶解玉米秸秆（10%，w/w）生成的 55.1±1.0g 糖中，可产生约 26.4±0.1g 乙醇，其中仅生成 0.4±0.0g 糠醛和 0.1±0.0g 羟甲基糠醛。
