Shanghai and Qingdao, People's Republic of China; and Tokyo, Japan From the Department of Plastic Surgery, Changzheng Hospital, Second Military Medical University; the Department of Hand Surgery, Hospital 401 of the People's Liberation Army; the Department of Anatomy, Second Military Medical University; and the Department of Plastic and Reconstructive Surgery, Keio University Hospital, Keio University.
Plast Reconstr Surg. 2012 Feb;129(2):401-411. doi: 10.1097/PRS.0b013e31823aeaae.
The arrangement and relationship of intramuscular nerves and blood vessels are critical to clinical physicians, but the majority of previous studies could not reflect them precisely.
In method 1, after rabbits were perfused with barium sulfate liquid silica gel, the specimen muscle was isolated and subjected to Sihler staining. The specimen muscles then underwent optical photography and molybdenum target radiography. The obtained photograph and the radiograph were then overlapped together to draw a map of intramuscular nerves and blood vessels. In method 2, after rabbits and child cadavers were perfused with semitransparent red liquid silica gel, the specimen muscle was isolated and subjected to Sihler staining. Finally, the specimen muscles were placed on an x-ray film viewer for photography.
Both methods had ideal outcomes. Through digital subtraction angiography, a distribution map of intramuscular nerves and blood vessels could be obtained in method 1, whereas in the photographs taken through method 2, the intramuscular nerves were counterstained with dark blue and the intramuscular blood vessels were stained red, which was more precise and direct. In method 2, specimens could be made into stereoscopic models.
These two methods that can simultaneously display intramuscular nerves and blood vessels have been significantly improved and lead to a good result. They also have their own advantages. When a muscle studied is small or flat, method 2 is recommended because of its conciseness and convenience. If the muscle is large, method 1 is applicable.
肌内神经和血管的排列和关系对临床医生至关重要，但大多数先前的研究无法准确反映这些关系。
方法 1 中，在使用硫酸钡液体硅胶灌注兔子后，分离标本肌肉并进行 Sihler 染色。然后对标本肌肉进行光学摄影和钼靶射线照相。将获得的照片和射线照片重叠在一起，以绘制肌内神经和血管的图谱。方法 2 中，在使用半透明红色液体硅胶灌注兔子和儿童尸体后，分离标本肌肉并进行 Sihler 染色。最后，将标本肌肉放置在 X 射线胶片观察器上进行摄影。
两种方法都取得了理想的结果。通过数字减影血管造影，可以在方法 1 中获得肌内神经和血管的分布图，而在方法 2 拍摄的照片中，肌内神经被染成深蓝色，肌内血管被染成红色，更加精确和直观。在方法 2 中，可以将标本制成立体模型。
这两种能够同时显示肌内神经和血管的方法都得到了显著改进并取得了良好的效果。它们各有优势。当研究的肌肉较小或较平平时，由于其简洁方便，建议使用方法 2。如果肌肉较大，则适用方法 1。
