Shin Kang-Jae, Yoo Ja-Young, Lee Ju-Young, Gil Young-Chun, Kim Jeong-Nam, Koh Ki-Seok, Song Wu-Chul
Department of Anatomy, Research Institute of Medical Science, Konkuk University School of Medicine, Seoul, Korea.
Department of Biomedical Laboratory Science, Masan University, Masan, Korea.
Anat Cell Biol. 2015 Dec;48(4):268-74. doi: 10.5115/acb.2015.48.4.268. Epub 2015 Dec 21.
The purposes of this study were therefore to characterize the degeneration and regeneration of nerves to the calf muscles after selective neurectomy, both macroscopically and microscopically, and to determine the incidence of such regeneration in a rabbit model. Seventy four New Zealand white rabbits were used. Selective neurectomy to the triceps surae muscles was performed, and the muscles were subsequently harvested and weighed 1-4 months postneurectomy. The gastrocnemius muscles were stained with Sihler's solution to enable the macroscopic observation of any nerve regeneration that may have occurred subsequent to neurectomy. The change in triceps surae muscle weight was measured along the time course of the experiment. After neurectomy, nerve degeneration was followed by regeneration in all cases. The weight of the triceps surae muscle decreased dramatically between completion of the neurectomy and 1 month postneurectomy, but increased thereafter. The nerve branches were weakly stained with Sihler's solution until 2 months postneurectomy, and then strongly stained after 3 months. The number of myelinated axons was decreased at 2 month after neurectomy compared to nonneurectomized controls, but then gradually increased thereafter. Although there are currently no reports on the incidence of recovery after calf reduction, it may be a very common occurrence in the clinical field based on our findings. The findings of this study provide fundamental anatomical and surgical information to aid planning and practice in calf-reduction surgery.
因此，本研究的目的是从宏观和微观层面描述选择性神经切除术后小腿肌肉神经的退化和再生情况，并确定兔模型中这种再生的发生率。使用了74只新西兰白兔。对腓肠肌进行选择性神经切除，随后在神经切除术后1至4个月收获肌肉并称重。用西勒氏溶液对腓肠肌进行染色，以便宏观观察神经切除术后可能发生的任何神经再生情况。在实验过程中测量腓肠肌重量的变化。神经切除术后，所有病例均先出现神经退化，随后再生。腓肠肌重量在神经切除完成后至神经切除术后1个月之间急剧下降，但此后有所增加。神经分支在神经切除术后2个月时用西勒氏溶液染色较弱，3个月后染色较强。与未进行神经切除的对照组相比，神经切除术后2个月时髓鞘轴突数量减少，但此后逐渐增加。尽管目前尚无关于小腿缩小术后恢复发生率的报道，但根据我们的研究结果，这在临床领域可能是非常常见的情况。本研究结果提供了基本的解剖学和手术信息，有助于小腿缩小手术的规划和实践。
