Inmunología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina.
Braz J Med Biol Res. 2012 Feb;45(2):139-46. doi: 10.1590/s0100-879x2012007500012. Epub 2012 Feb 2.
The aim of the present study was to determine whether lipoarabinomannan (LAM), in combination with Freund's incomplete adjuvant (FIA), was able to improve cell-mediated and antibody-mediated immune responses against ovalbumin (OVA) in cattle. Twenty-three calves were assigned to four treatment groups, which were subcutaneously immunized with either OVA plus FIA, OVA plus FIA and LAM from Mycobacterium avium subsp avium, FIA plus LAM, or FIA alone. Lymphoproliferation, IFN-γ production and cell subpopulations on peripheral blood mononuclear cells before and 15 days after treatment were evaluated. Delayed hypersensitivity was evaluated on day 57. Specific humoral immune response was measured by ELISA. Inoculation with LAM induced higher levels of lymphoproliferation and IFN-γ production in response to ConA and OVA (P < 0.05). Specific antibody titers were similar in both OVA-immunized groups. Interestingly, our results showed that the use of LAM in vaccine preparations improved specific cell immune response evaluated by lymphoproliferation and IFN-γ production by at least 50 and 25%, respectively, in cattle without interfering with tuberculosis and paratuberculosis diagnosis.
本研究旨在确定脂阿拉伯甘露聚糖（LAM）与弗氏不完全佐剂（FIA）联合使用是否能提高牛对卵清蛋白（OVA）的细胞介导和抗体介导免疫应答。将 23 头小牛分配到四个治疗组中，这些小牛分别接受以下方式的皮下免疫接种：OVA 加 FIA、OVA 加禽分枝杆菌来源的 LAM、FIA 加 LAM 或单独 FIA。在治疗前和治疗后 15 天评估淋巴细胞增殖、IFN-γ 产生和外周血单核细胞的细胞亚群。在第 57 天评估迟发型超敏反应。通过 ELISA 测量特异性体液免疫应答。接种 LAM 可诱导针对 ConA 和 OVA 的淋巴细胞增殖和 IFN-γ 产生更高水平（P <0.05）。OVA 免疫组的特异性抗体滴度相似。有趣的是，我们的结果表明，在不干扰结核病和副结核病诊断的情况下，LAM 用于疫苗制剂可分别将特异性细胞免疫应答的淋巴细胞增殖和 IFN-γ 产生至少提高 50%和 25%。
