通过染料配体亲和色谱法鉴定的核苷酸配体对结晶的增强作用。
Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography.
作者信息
Kim Heungbok, Webster Cecelia, Roberts Justin K M, Kositsawat Juthamas, Hung Li-Wei, Terwilliger Thomas C, Kim Chang-Yub
机构信息
Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
出版信息
J Struct Funct Genomics. 2012 Jun;13(2):71-9. doi: 10.1007/s10969-012-9124-8.
Ligands interacting with Mycobacterium tuberculosis recombinant proteins were identified through use of the ability of Cibacron Blue F3GA dye to interact with nucleoside/nucleotide binding proteins, and the effects of these ligands on crystallization were examined. Co-crystallization with ligands enhanced crystallization and enabled X-ray diffraction data to be collected to a resolution of atleast 2.7 Å for 5 of 10 proteins tested. Additionally, clues about individual proteins’ functions were obtained from their interactions with each of a panel of ligands.
利用汽巴克隆蓝F3GA染料与核苷/核苷酸结合蛋白相互作用的能力，鉴定了与结核分枝杆菌重组蛋白相互作用的配体，并研究了这些配体对结晶的影响。与配体共结晶增强了结晶效果，并使所测试的10种蛋白质中的5种能够收集到分辨率至少为2.7 Å的X射线衍射数据。此外，通过单个蛋白质与一组配体中每种配体的相互作用，获得了有关其功能的线索。
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