University of Utah School of Medicine, Division of Otolaryngology-Head & Neck Surgery, Salt Lake City, UT, USA.
Int Forum Allergy Rhinol. 2011 Mar-Apr;1(2):101-5. doi: 10.1002/alr.20031.
In chronic rhinosinusitis (CRS), the inflammation leads to a proliferative response in the extracellular matrix (ECM). Periostin and osteopontin are 2 ECM proteins which have received attention for their roles in tissue remodeling in inflammatory diseases of the upper and lower airways. Transforming growth factor beta-1 (TGFβ1) is an inflammatory cytokine that has been implicated in fibrotic conditions affecting virtually every organ. In this study we seek to evaluate the differential expression of periostin, osteopontin, and TGFβ1 in the ethmoid sinus and nasal floor of patients with CRS. Furthermore, we seek to determine if a correlation exists between their differential expression in the nose and sinuses of patients with CRS.
Biopsies from ethmoid sinus and nasal floor mucosa were taken from 15 patients undergoing functional endoscopic sinus surgery (FESS) for CRS. Complementary DNA (cDNA) microarray analysis demonstrated upregulation of periostin and osteopontin in the ethmoid sinus samples. Quantitative real-time polymerase chain reaction (RT-PCR) was performed for periostin, osteopontin, and TGFβ1. Statistical analysis was undertaken to examine correlations between the 3 genes of interest.
RT-PCR confirmed that periostin and osteopontin were overexpressed in the ethmoid samples compared to the nasal floor. The differential expression of both periostin and osteopontin showed significant correlation with TGFβ1.
Periostin and osteopontin appear to be involved in ECM remodeling seen in CRS. TGFβ1 may be an upstream inducer of CRS-related changes in ethmoid sinus mucosa.
在慢性鼻-鼻窦炎（CRS）中，炎症导致细胞外基质（ECM）的增殖反应。骨膜蛋白和骨桥蛋白是 2 种 ECM 蛋白，它们在上下呼吸道炎症性疾病中的组织重塑作用受到关注。转化生长因子-β1（TGFβ1）是一种炎症细胞因子，与影响几乎所有器官的纤维化疾病有关。在这项研究中，我们试图评估骨膜蛋白、骨桥蛋白和 TGFβ1 在 CRS 患者筛窦和鼻底的差异表达。此外，我们试图确定它们在 CRS 患者鼻腔和鼻窦中的差异表达之间是否存在相关性。
从 15 例行功能性内镜鼻窦手术（FESS）治疗 CRS 的患者的筛窦和鼻底黏膜中采集活检组织。互补 DNA（cDNA）微阵列分析显示骨膜蛋白和骨桥蛋白在上筛窦样本中上调。进行了骨膜蛋白、骨桥蛋白和 TGFβ1 的定量实时聚合酶链反应（RT-PCR）。进行了统计学分析，以检查 3 个感兴趣基因之间的相关性。
RT-PCR 证实，与鼻底相比，筛窦样本中骨膜蛋白和骨桥蛋白表达过度。骨膜蛋白和骨桥蛋白的差异表达与 TGFβ1 呈显著相关性。
骨膜蛋白和骨桥蛋白似乎参与了 CRS 中所见的 ECM 重塑。TGFβ1 可能是 CRS 相关筛窦黏膜变化的上游诱导剂。
