Sirca Saša, Urek Gregor, Lazarova Stela, Elshishka Milka, Peneva Vlada
Agricultural Institute of Slovenia, Hacquetova ulica 17, 1001 Ljubljana, Slovenia.
Zookeys. 2011(141):1-27. doi: 10.3897/zookeys.141.1906. Epub 2011 Oct 28.
A new needle nematode, Longidorus carniolensissp. n., recovered from the soil around the roots of grapevine Vitis vinifera L. from Slovenia, is described and illustrated. Longidorus carniolensisis an amphimictic species, characterised by females with a moderately long (L=5.6-8.2 mm) and plump (a=51-72.4, ave. 66.3) body, assuming a spiral to C-shape when heat relaxed. Head region continuous, anteriorly almost flat, lip region 23-25 µm wide; guiding ring situated posteriorly (42-47 μm, 43-50 μm in males), odontostyle long (ave. 146.6 (136-157) μm); pharyngeal glands with normal location, their nuclei of approximately equal size; tail bluntly conoidal to almost hemispherical. Males abundant, spicules slender and long (122-145 μm), ventromedian supplements 13-17, irregularly spaced, preceded by an adanal pair. Four juvenile stages present, the first stage juvenile with bluntly conoidal tail. Codes for identifying the new species when using the key by Chen et al. (1997) are: A 56, B 4, C 4, D 1, E 4, F 35, G 1, H 1, I 2. The new species is morphologically the most similar to Longidorus poessneckensis Altherr, 1974, Longidorus macrosoma Hooper, 1961, Longidorus caespiticola Hooper, 1961, Longidorus helveticus Lamberti et al., 2001, Longidorus macroteromucronatus Altherr, 1974, Longidorus pius Barsi & Lamberti, 2001, Longidorus raskii Lamberti & Agostinelli, 1993, Longidorus kheirii Pedram et al. 2008, Longidorus silvae Roca, 1993, Longidorus iuglandis Roca et al., 1985, Longidorus vinearum Bravo & Roca, 1995 and Longidorus major Roca & d'Erico, 1987, but differs from these species either by the body and odontostyle length, position of guide ring, head region and tail shape or the shape of the first stage juvenile tail. Sequence data from the D2-D3 region of the 28S rDNA distinguishes this new species from other speciesof the genus Longidorus with known sequences. Relationships of Longidorus carniolensissp. n. with other Longidorus species based on analysis of this DNA fragment and morphology are discussed.
本文描述并图示了一种从斯洛文尼亚葡萄（Vitis vinifera L.）根系周围土壤中分离得到的新针属线虫——卡尼奥拉针线虫（Longidorus carniolensissp. n.）。卡尼奥拉针线虫是两性生殖物种，其雌虫特征为身体中等长度（L = 5.6 - 8.2毫米）且饱满（a = 51 - 72.4，平均66.3），热松弛时呈螺旋形至C形。头部区域连续，前部几乎扁平，唇区宽23 - 25微米；引导环位于后部（42 - 47微米，雄虫为43 - 50微米），口针长（平均146.6（136 - 157）微米）；咽腺位置正常，其细胞核大小近似；尾部钝锥形至近半球形。雄虫数量众多，交合刺细长（122 - 145微米），腹中线补充器13 - 17个，间距不规则，前有一对肛侧补充器。有四个幼虫阶段，第一阶段幼虫尾部钝锥形。使用Chen等人（1997年）的检索表鉴定该新物种的代码为：A 56，B 4，C 4，D 1，E 4，F 35，G 1，H 1，I 2。该新物种在形态上与1974年的波斯内克针线虫（Longidorus poessneckensis Altherr）、1961年的大针线虫（Longidorus macrosoma Hooper）、1961年的丛生针线虫（Longidorus caespiticola Hooper）、2001年的瑞士针线虫（Longidorus helveticus Lamberti et al.）、1974年的大端突针线虫（Longidorus macroteromucronatus Altherr）、2001年的皮尤斯针线虫（Longidorus pius Barsi & Lamberti）、1993年的拉斯基针线虫（Longidorus raskii Lamberti & Agostinelli）、2008年的凯尔针线虫（Longidorus kheirii Pedram et al.）、1993年的西尔瓦针线虫（Longidorus silvae Roca）、1985年的胡桃针线虫（Longidorus iuglandis Roca et al.）、1995年的葡萄针线虫（Longidorus vinearum Bravo & Roca）以及1987年的大针线虫（Longidorus major Roca & d'Erico）最为相似，但在身体和口针长度、引导环位置、头部区域和尾部形状或第一阶段幼虫尾部形状等方面与这些物种存在差异。基于28S rDNA的D2 - D3区域的序列数据可将该新物种与针属其他已知序列的物种区分开来。本文还讨论了基于该DNA片段分析和形态学的卡尼奥拉针线虫与其他针属物种的关系。
