Barsalote Eda Marie, Pham Hoa Thi, Lazarova Stela, Peneva Vlada, Zheng Jingwu
Laboratory of Plant Nematology, Institute of Biotechnology, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, China.
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Street, 1113 Sofia, Bulgaria.
Zookeys. 2018 Mar 19(744):1-18. doi: 10.3897/zookeys.744.23265. eCollection 2018.
., an amphimictic species recovered from the rhizosphere of and in Shanxi and Beijing, China, is described and illustrated. The taxonomic position of among other species within the genus was elucidated using morphometric and molecular data, and phylogenetic relationships were inferred using D2-D3 expansion domains of 28S and 18S rRNA genes by Bayesian Inference (BI) method. The new species is characterised by females with a medium body size (L = 4.9-6.6 mm), a lip region slightly expanded, broadly rounded frontally and laterally, the amphidial fovea broad and symmetrically bilobed at base, odontostyle long and slender (143-168 μm), odonthophore slightly swollen at the base, tail short bluntly conoid to rounded. Guide ring located far posterior from the oral aperture (70-93 μm). Males with two ad-cloacal pairs of supplements preceded by a row of 10-14 ventromedian supplements, with robust spicules measuring 111-126 μm along the median line. Three juvenile stages were present, tail shape of J1 elongate conoid while in J2 and J3 the tail gradually becomes bluntly rounded. Codes for identifying the new species are: A6-B3-C5-D2-E2-F3-G1-H1-I2-J2-K2. belongs to a group of species with a guide ring at the mid-odontostyle position that have a predominantly Asiatic origin. It differs from all of them by a combination of morphological characters and unique sequences of partial 18S and D2-D3 region of 28S rRNA genes. The percentage dissimilarities in partial 18S and D2-D3 28S rRNA genes of to the closest species (, , and ) were 1.5 %-1.8 % and 16.8-18.3 %, respectively.
本文描述并阐释了一种两性生殖的物种，该物种是从中国山西和北京的[植物名称1]和[植物名称2]根际分离得到的。利用形态测量和分子数据阐明了该物种在属内其他物种中的分类地位，并通过贝叶斯推断（BI）方法，使用28S和18S rRNA基因的D2 - D3扩展区域推断系统发育关系。新物种的特征为：雌虫体型中等（体长L = 4.9 - 6.6毫米），唇区稍膨大，前端和侧面宽圆形，头感器窝基部宽且对称双叶状，口针长而细（143 - 168微米），口针基部稍肿胀，尾短，钝圆锥形至圆形。引导环位于口孔后方较远位置（70 - 93微米）。雄虫有两对泄殖腔前附器，之前有一排10 - 14个腹中线附器，强壮的交合刺沿中线长111 - 126微米。存在三个幼虫阶段，J1的尾形为细长圆锥形，而J2和J3的尾逐渐变为钝圆形。鉴定新物种的编码为：A6 - B3 - C5 - D2 - E2 - F3 - G1 - H1 - I2 - J2 - K2。该物种属于在口针中部位置有引导环的一组物种，主要起源于亚洲。它与所有这些物种的区别在于形态特征组合以及28S rRNA基因部分18S和D2 - D3区域的独特序列。该物种与最接近的物种（[物种名称1]、[物种名称2]、[物种名称3]和[物种名称4]）在28S rRNA基因部分18S和D2 - D3区域的差异百分比分别为1.5% - 1.8%和16.8 - 18.3%。
