Department of Pharmacology, Xi'an Medical University, Xi'an, Shaanxi, PR China.
Eur Rev Med Pharmacol Sci. 2011 Dec;15(12):1452-60.
Oral administration of selenium is an effective treatment for diabetes in animal models. However, selenium exerts these effects at high doses and several toxic effects are produced. Low doses of selenium are relatively safe but are unable to elicit any antidiabetic effect.
The present study explored the prospect of using low doses of insulin in combination with selenium to evaluate their antidiabetic effect, and to evaluate their effect on IRS-1, PI3K and GLUT4 levels in cardiac muscle of diabetic rats.
Diabetic rats were treated with insulin, selenium, and insulin and selenium in combination for four weeks. The effect of these antidiabetic compounds was examined on general physiological parameters and distribution of IRS-1, PI3K and GLUT4 in cardiac muscle by immunoblotting and immunohistochemistry.
Insulin in combination with selenium could significantly revive normoglycemia, and restore the disturbances in IRS-1, PI3K and GLUT4 levels in cardiac muscle. Treatment with insulin was only partially effective in the restoration of diabetic alterations.
The treatment of diabetic rats with combined doses of insulin and selenium was most effective in controlling glycaemia, and remarkably restored GLUT4 distribution by IRS-1/PI3K-dependent pathway in cardiac muscle of diabetic rat.
口服硒是治疗动物模型糖尿病的有效方法。然而，硒在高剂量下发挥作用，并产生多种毒性作用。低剂量的硒相对安全，但不能产生任何抗糖尿病作用。
本研究探讨了低剂量胰岛素与硒联合应用的前景，以评估其在糖尿病大鼠心肌中对 IRS-1、PI3K 和 GLUT4 水平的影响。
用胰岛素、硒和胰岛素与硒联合治疗糖尿病大鼠 4 周。通过免疫印迹和免疫组化检测这些抗糖尿病化合物对一般生理参数和 IRS-1、PI3K 和 GLUT4 在心肌中分布的影响。
胰岛素与硒联合使用可显著恢复正常血糖，并恢复糖尿病大鼠心肌中 IRS-1、PI3K 和 GLUT4 水平的紊乱。胰岛素治疗仅部分有效恢复糖尿病的改变。
用胰岛素和硒联合治疗糖尿病大鼠对控制血糖最有效，并通过 IRS-1/PI3K 依赖途径显著恢复糖尿病大鼠心肌中 GLUT4 的分布。
