电针通过激活股四头肌中的SIRT1和GLUT4改善高脂饮食诱导的胰岛素抵抗大鼠的胰岛素敏感性。

ELECTROACUPUNCTURE IMPROVES INSULIN SENSITIVITY IN HIGH-FAT DIET-INDUCED INSULIN RESISTANT RATS BY ACTIVATING SIRT1 AND GLUT4 IN QUADRICEPS FEMORIS.

作者信息

Xu F, Chen R, Ma C, Tang L, Wan W, You F, Chen L, Li J, Chen Z, Liang F

机构信息

Wuhan, China.

Hubei University of Chinese Medicine, Department of Acupuncture and Moxibustion, Wuhan, China.

出版信息

Acta Endocrinol (Buchar). 2020 Jul-Sep;16(3):280-287. doi: 10.4183/aeb.2020.280.

DOI:10.4183/aeb.2020.280

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7748239/

Abstract

OBJECTIVES

To assess the effects of electroacupuncture (EA) at the Zusanli (ST36), Guanyuan (CV4), Zhongwan (CV12), and Fenglong (ST40) acupoints on sirtuin 1 (SIRT1) and glucose transporter type 4 (GLUT4) expression in high-fat diet (HFD)-induced insulin-resistant (IR) rats.

METHODS

Wistar rats were divided into normal control (NC), HFD, and HFD+EA groups. NC rats were fed a standard chow diet and did not receive EA. After being fed an HFD for eight weeks, rats in the HFD+EA group received EA at 2 Hz five times a week for eight weeks. Rats in the HFD group did not receive EA.

RESULTS

In HFD-induced IR rats, EA inhibited body weight increase and water intake, which were observed in HFD rats. EA had no effect on fasting blood glucose and postprandial blood sugar levels. Intraperitoneal insulin tolerance testing revealed that EA enhanced insulin sensitivity in HFD-induced IR rats. Compared with NC rats, SIRT1 and GLUT4 were downregulated in the quadriceps femoris of HFD-fed rats but were increased after eight weeks of EA stimulation.

CONCLUSIONS

EA enhanced HFD-induced insulin resistance by activating SIRT1 and GLUT4 in the quadriceps femoris. These results provide powerful evidence supporting the beneficial effects of EA on HFD-induced insulin resistance.

摘要

目的

评估针刺足三里（ST36）、关元（CV4）、中脘（CV12）和丰隆（ST40）穴位对高脂饮食（HFD）诱导的胰岛素抵抗（IR）大鼠中沉默调节蛋白1（SIRT1）和葡萄糖转运蛋白4（GLUT4）表达的影响。

方法

将Wistar大鼠分为正常对照组（NC）、高脂饮食组（HFD）和高脂饮食+电针组（HFD+EA）。NC组大鼠喂食标准饲料，不接受电针治疗。高脂饮食组大鼠喂食高脂饲料8周后，HFD+EA组大鼠每周接受5次2Hz的电针刺激，持续8周。HFD组大鼠不接受电针治疗。

结果

在高脂饮食诱导的胰岛素抵抗大鼠中，电针抑制了高脂饮食大鼠出现的体重增加和饮水量增加。电针对空腹血糖和餐后血糖水平无影响。腹腔胰岛素耐量试验显示，电针增强了高脂饮食诱导的胰岛素抵抗大鼠的胰岛素敏感性。与NC组大鼠相比，高脂饮食喂养大鼠股四头肌中的SIRT1和GLUT4表达下调，但在电针刺激8周后升高。

结论

电针通过激活股四头肌中的SIRT1和GLUT4增强了高脂饮食诱导的胰岛素抵抗。这些结果为电针治疗高脂饮食诱导的胰岛素抵抗的有益作用提供了有力证据。

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