Rikimaru K, Toda H, Tachikawa N, Kamata N, Enomoto S
Second Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
In Vitro Cell Dev Biol. 1990 Sep;26(9):849-56. doi: 10.1007/BF02624609.
A novel protein-free synthetic medium has been developed for the culture of human squamous cell carcinoma cells. This medium, designated PF86-1, supports the serial subcultivation of six out of nine human squamous cell carcinoma cell lines in a protein-free, chemically defined condition without the adapting culture from serum-containing conditions. These cell lines growing in PF86-1 exhibited nearly equal potency to grow in massive culture without noticeable changes in morphology but presented a significantly decreased level of colony forming efficiency when compared with the cells cultured in serum-containing media, suggesting the implication of some autocrine mechanism. Interestingly, this medium supported the growth of normal human squamous cells of oral mucosa and skin for more than 2 mo. in the primary explant culture in spite of high levels of calcium ion concentration, where the overgrowth of fibroblasts as contaminant was not observed. These results suggest that PF86-1 supports the growth of cells derived from epidermal tissues selectively and provides the same defined condition for growth of malignant and nonmalignant human squamous cells. It seems, therefore, that PF86-1 allows investigations on the products of squamous cell carcinoma cells or on the differences of growth mechanisms between normal and neoplastic human squamous cells.
已开发出一种新型无蛋白合成培养基用于培养人鳞状细胞癌细胞。这种培养基命名为PF86 - 1，可在无蛋白、化学成分明确的条件下支持9种人鳞状细胞癌细胞系中的6种进行连续传代培养，无需从含血清条件下进行适应性培养。在PF86 - 1中生长的这些细胞系在大规模培养中表现出几乎相同的生长能力，形态无明显变化，但与在含血清培养基中培养的细胞相比，集落形成效率显著降低，提示存在某种自分泌机制。有趣的是，尽管钙离子浓度较高，但这种培养基在原代外植体培养中支持口腔黏膜和皮肤的正常人鳞状细胞生长超过2个月，未观察到成纤维细胞作为污染物过度生长的情况。这些结果表明，PF86 - 1选择性地支持源自表皮组织的细胞生长，并为恶性和非恶性人鳞状细胞的生长提供相同的明确条件。因此，PF86 - 1似乎可用于研究鳞状细胞癌细胞的产物或正常与肿瘤性人鳞状细胞之间生长机制的差异。
