National Dairy Research Institute, Karnal, Haryana, India.
J Anim Physiol Anim Nutr (Berl). 2013 Apr;97(2):315-21. doi: 10.1111/j.1439-0396.2011.01272.x. Epub 2012 Jan 31.
To study the effect of vitamin E (VE), copper (Cu) and zinc (Zn) supplementation on the in vitro phagocytic activity (PA) and lymphocyte proliferation response (LPR) of blood neutrophils and lymphocytes, thirty Sahiwal pregnant cows (six in each group) in their late gestation at 30 days before the expected date of calving were selected from the NDRI experimental herd and supplemented with various micronutrients from 30 days before calving to 45 days after calving. Cows were supplemented individually with VE (1000 IU/cow/day), Cu (20 ppm/cow/day) and Zn (80 ppm/cow/day) and also with a combination of VE, Cu and Zn to study cumulative effect of all micronutrients. One group without any supplementation acted as a control. Blood neutrophils and lymphocytes were isolated and studied for their PA and LPR. Supplementation of micronutrients like VE, Cu, Zn and a combination of all these nutrients significantly (p < 0.01) increased the PA of experimental cows as compared to control (unsupplemented) cows during the pre-partum period. During post-partum, all the micronutrients (VE, Cu, Zn and their combination) showed a significant (p < 0.01) increase in the PA of experimental cows as compared to control cows. Of all the groups, significant (p < 0.01) and maximum PA was observed in the combination group followed by Zn-supplemented group during both the pre- and post-partum period. A significant (p < 0.01) increase in LPR of B lymphocytes was observed in combination-supplemented group during the pre-partum period and during both the pre- and post-partum period in the Cu-supplemented group.
为了研究维生素 E（VE）、铜（Cu）和锌（Zn）补充剂对血液中性粒细胞和淋巴细胞体外吞噬活性（PA）和淋巴细胞增殖反应（LPR）的影响，从 NDRI 实验牛群中选择了 30 头萨希瓦尔怀孕奶牛（每组 6 头），这些奶牛处于妊娠晚期，在预计分娩前 30 天开始，直至分娩后 45 天，一直补充各种微量营养素。奶牛单独补充 VE（1000 IU/头/天）、Cu（20 ppm/头/天）和 Zn（80 ppm/头/天），并补充 VE、Cu 和 Zn 的组合，以研究所有微量营养素的累积效应。一组不补充任何营养素作为对照。分离血液中性粒细胞和淋巴细胞，研究其 PA 和 LPR。与对照组（未补充）相比，VE、Cu、Zn 以及这些营养素的组合补充剂在产前期间显著（p<0.01）增加了实验奶牛的 PA。在产后期间，与对照组相比，所有微量营养素（VE、Cu、Zn 及其组合）均显著（p<0.01）增加了实验奶牛的 PA。在所有组中，组合组在产前和产后期间均观察到显著（p<0.01）和最大的 PA，其次是 Zn 补充组。在产前期间，组合补充组的 B 淋巴细胞 LPR 显著（p<0.01）增加，Cu 补充组在产前和产后期间均观察到显著（p<0.01）增加。
