Department of Chemistry, Tamkang University, Tamsui, New Taipei City, Taiwan.
Drug Chem Toxicol. 2012 Jul;35(3):341-6. doi: 10.3109/01480545.2011.622771. Epub 2012 Jan 31.
We used ultraviolet (UV)-induced fin damage in zebrafish as a system for evaluating the chemopreventive potential of flavonoids. Chemopreventive effects of each compound, including flavone, flavanone, and chalcone, on fin development were evaluated using Kaplan-Meier analysis and Cox proportional hazards regression. Results showed that 1) flavone has the highest capacity to protect zebrafish fins from UV-induced damages among other groups; 2) zebrafish fins in the UV+1 ppm flavone group are 1.029.60 times more likely to return to normal fins than ones in the UV-only group, but fins in the UV+20 ppm flavone group are only 0.455.66 times more likely to recover; and 3) flavone significantly reduced ROS production in UV-exposed zebrafish embryos, which may attenuate UV-mediated apoptosis. In conclusion, zebrafish can be used as a system for comparing the UV-protection efficacy of flavonoids.
我们利用斑马鱼的紫外线（UV）诱导的鳍损伤作为评估类黄酮化学预防潜力的系统。使用 Kaplan-Meier 分析和 Cox 比例风险回归评估每种化合物（包括黄酮、黄烷酮和查耳酮）对鳍发育的化学预防作用。结果表明：1）与其他组相比，黄酮具有最高的保护斑马鱼鳍免受 UV 损伤的能力；2）与仅 UV 组相比，UV+1 ppm 黄酮组的斑马鱼鳍恢复正常鳍的可能性高 1.029.60 倍，但 UV+20 ppm 黄酮组的鳍恢复正常的可能性仅高 0.455.66 倍；3）黄酮显著减少了暴露于 UV 的斑马鱼胚胎中的 ROS 产生，这可能会减轻 UV 介导的细胞凋亡。总之，斑马鱼可作为比较类黄酮的 UV 保护功效的系统。
