Graduate Institute of Life Sciences, Tamkang University, Tamsui, Taiwan.
Toxicol Mech Methods. 2011 Jan;21(1):63-9. doi: 10.3109/15376516.2010.529191. Epub 2010 Nov 10.
This study applied broccoli and cauliflower extracts (whole, floret, and stem) to zebrafish larvae in parallel to receive 100 mJ/cm(2) of UVB six times, and recorded their fin malformation phenotypes. Chemopreventive effects of each group, including UVB, whole-, floret-, and stem-extracts of broccoli and cauliflower on fin development were evaluated using Kaplan-Meier analysis, log-rank test, and Cox proportional hazards regression. Results showed that (1) zebrafish fins in the UVB + whole broccoli extract group are 6.209.32-times more likely to return to normal fins than ones in the UVB only group, but fins in the UVB + whole cauliflower extract group are only 5.1311.10-times more likely to recover, indicated that whole broccoli and cauliflower extract had similar chemopreventive ability on fin development; and (2) the broccoli stem has the highest antioxidant capacity among other groups. In conclusion, zebrafish can be used as a system for evaluating the efficacy of other UVB protective compounds.
本研究将西兰花和菜花提取物（整株、小花和茎）应用于斑马鱼幼体，同时接受 100 mJ/cm(2)的 UVB 照射六次，并记录其鳍畸形表型。使用 Kaplan-Meier 分析、对数秩检验和 Cox 比例风险回归评估每组（包括 UVB、西兰花整株、小花和茎提取物）对鳍发育的化学预防作用。结果表明：(1) 与仅接受 UVB 照射的组相比，UVB+整株西兰花提取物组的斑马鱼鳍恢复正常的可能性高 6.209.32 倍，但 UVB+整株菜花提取物组的鳍仅高 5.1311.10 倍，表明整株西兰花和菜花提取物对鳍发育具有相似的化学预防能力；(2) 与其他组相比，西兰花茎具有最高的抗氧化能力。总之，斑马鱼可用于评估其他 UVB 保护化合物功效的系统。
