Joseleau-Petit D, Kepes F, Peutat L, D'Ari R, Rothfield L I
Institut Jacques Monod, Université Paris VII, France.
J Bacteriol. 1990 Nov;172(11):6573-5. doi: 10.1128/jb.172.11.6573-6575.1990.
Synchronized cells of Escherichia coli were pulse-labeled with [3H]leucine and subjected to membrane fractionation to determine whether a fraction that is enriched for membrane-murein adhesion zones (fraction OML) was preferentially generated at specific times during the cell cycle, as previously suggested from studies of lkyD and cha mutants. Contrary to this prediction, the experiments showed that OML was formed continuously during the division cycle.
用[³H]亮氨酸对同步化的大肠杆菌细胞进行脉冲标记,并进行膜分级分离,以确定富含膜-胞壁质粘附区的级分(OML级分)是否如先前对lkyD和cha突变体的研究所暗示的那样,在细胞周期的特定时间优先产生。与这一预测相反,实验表明OML在分裂周期中持续形成。