Department of Microbiological Surveillance and Research, Statens Serum Institut, Copenhagen, Denmark.
Pediatr Res. 2012 Jan;71(1):115-20. doi: 10.1038/pr.2011.1.
The pathophysiology of necrotizing enterocolitis (NEC) is multifactorial, and gastrointestinal bacteria are thought to play an important role. In this study, the role of microflora in the gastrointestinal tract of neonates with NEC was assessed by comparing cases with controls.
Of the 163 neonates, 21 developed NEC. The risk of NEC decreased by 8% with each additional day of gestational age.
Typically, very few bacterial species could be cultured from the fecal specimens obtained. Gram-positive (G(+)) bacteria dominated the samples in the NEC group, whereas in the control group mixed flora of G(+) and Gram-negative (G(-)) bacteria were isolated. Surprisingly, molecular analysis using PCR-DGGE profiles did not confirm these differences. Our data suggest that G(+) bacteria in the intestine may play a role in the development of NEC in premature infants.
One hundred and sixty three neonates born at <30 weeks of gestation were enrolled. Fecal samples taken during the first month of life were subjected to culture and PCR-denaturing gradient gel electrophoresis (PCR-DGGE). A total of 482 fecal samples were examined.
坏死性小肠结肠炎（NEC）的发病机制是多因素的，胃肠道细菌被认为起着重要作用。在这项研究中，通过比较病例和对照组来评估胃肠道中微生物群在患有 NEC 的新生儿中的作用。
在 163 名新生儿中，有 21 名发生了 NEC。胎龄每增加一天，NEC 的风险就会降低 8%。
通常情况下，从获得的粪便标本中只能培养出很少的细菌种类。革兰氏阳性（G(+)）细菌在 NEC 组的样本中占主导地位，而在对照组中则分离出混合的 G(+)和革兰氏阴性（G(-））细菌。令人惊讶的是，使用 PCR-DGGE 图谱进行的分子分析并未证实这些差异。我们的数据表明，肠道中的 G(+)细菌可能在早产儿 NEC 的发展中起作用。
共纳入了 163 名胎龄<30 周的新生儿。在生命的第一个月中采集的粪便样本进行培养和 PCR-变性梯度凝胶电泳（PCR-DGGE）分析。共检查了 482 份粪便样本。
