Yokota E, Mabuchi I
Department of Biology, College of Arts and Sciences, University of Tokyo.
J Biochem. 1990 Jul;108(1):1-3. doi: 10.1093/oxfordjournals.jbchem.a123146.
When sea urchin spermatozoa were treated with a Triton X-100 solution, cAMP-dependent protein kinase (cA-kinase) activity was extracted. Further extraction with Triton X-100 of axonemes isolated from the Triton-extracted sperm again released a considerable amount of the cA-kinase activity. The activity which remained after extraction three times with Triton X-100 was released by treatment with a low salt solution. These activities found in the various extracts were likely to be due to the same cA-kinase, which was a mammalian type II-like enzyme. The cA-kinase activity that remained in the axonemes after the first Triton X-100 extraction may be involved in the regulation of flagellar movement in the Triton-extracted sperm.
当用Triton X-100溶液处理海胆精子时，可提取出环磷酸腺苷依赖性蛋白激酶（cA激酶）活性。用Triton X-100对从经Triton处理的精子中分离出的轴丝进行进一步提取，再次释放出相当数量的cA激酶活性。用Triton X-100提取三次后仍残留的活性，经低盐溶液处理后被释放出来。在各种提取物中发现的这些活性可能归因于同一种cA激酶，它是一种类似哺乳动物II型的酶。第一次用Triton X-100提取后残留在轴丝中的cA激酶活性可能参与了经Triton处理的精子中鞭毛运动的调节。
