Liu Xue, Abd El-Aty A M, Cho S-K, Yang Angel, Park Jong-Hyouk, Shim Jae-Han
Natural Products Chemistry Laboratory, College of Agriculture and Life Science, Chonnam National University, 300 Yong-Bong Dong, Buk-Ku, Gwangju, Republic of Korea.
Biomed Chromatogr. 2012 Oct;26(10):1157-62. doi: 10.1002/bmc.2671. Epub 2012 Jan 30.
The extraction and identification of bioactive compounds from herbs is of great interest. In this study, accelerated solvent extraction (ASE) technique was used to analyze the secondary volatile profiles in Nigella sativa seeds obtained from two different origins, Egypt and Bangladesh. The main extraction parameters, including extraction temperature, pressure and static extraction time, were investigated and optimized. Identification and quantification of the major constituents in nonpolar extracts (hexane) were achieved by means of GC-FID/GC-MS analysis with external standards. The two seeds showed a similar variety of chemical composition; however, the secondary volatiles profile of Bangladesh seed was higher than that of the Egyptian seed. A total of 25 compounds were identified from the ASE extract under the following optimum extraction conditions: 100°C, 1500 psi and 5 min, for extraction temperature, pressure and static time, respectively. The proposed technique can be used for the characterization of N. sativa varieties or cultivars.
从草药中提取和鉴定生物活性化合物备受关注。在本研究中，采用加速溶剂萃取（ASE）技术分析了来自埃及和孟加拉国两个不同产地的黑种草种子中的次生挥发性成分。研究并优化了主要萃取参数，包括萃取温度、压力和静态萃取时间。通过外标法的气相色谱 - 火焰离子化检测器/气相色谱 - 质谱联用（GC - FID/GC - MS）分析实现了非极性提取物（己烷）中主要成分的鉴定和定量。两种种子显示出相似的化学成分种类；然而，孟加拉国种子的次生挥发性成分谱高于埃及种子。在以下最佳萃取条件下，分别为萃取温度100°C、压力1500 psi和静态时间5分钟，从ASE提取物中总共鉴定出25种化合物。所提出的技术可用于黑种草品种或栽培品种的表征。
