Department of Food Science, Research Centre Aarslev, Aarhus University, Kirstinebjergvej 10, DK-5792 Aarslev, Denmark.
Magn Reson Chem. 2011 Dec;49 Suppl 1:S71-8. doi: 10.1002/mrc.2805.
In the present study, meat obtained from uncommon and novel pig crossings between the rare Iberian and Mangalitza pigs and the more frequent Duroc and Landrace/Yorkshire pigs was characterized by time-domain proton NMR relaxometry and high-resolution proton NMR spectroscopy to elucidate the potential of NMR to assess the meat quality of new-introduced pig breeds. Multivariate data analysis of proton NMR T(2) relaxation curves obtained on fresh meat samples revealed differences in the T(2) relaxation pattern of the different breeds included in the study. Comparison of NMR T(2) relaxation data with gravimetric determination of water-holding capacity (WHC) indicated that this should be ascribed to differences in the WHC of the different meats, and that NMR T(2) relaxation in accordance with previous studies provides unique information about WHC, which may be ascribed to the fact that NMR T(2) relaxation reflects information about intrinsic meat structure. High-resolution proton NMR spectroscopy of freeze exudate and meat extracts also revealed differences in the metabolite profile of the meat between the different breeds studied. The effects of breed on the amount of lactate in the freeze exudate were observed, which could be linked to WHC of the meat. In conclusion, the different NMR techniques applied could provide complementary information about biophysical and biochemical factors of importance for meat quality assessment.
在本研究中,通过时域质子 NMR 弛豫度测定法和高分辨率质子 NMR 波谱法对来自稀有伊比利亚猪和曼加利察猪与更为常见的杜洛克猪和长白猪/约克夏猪之间的新型猪杂交品种的肉进行了分析,以阐明 NMR 用于评估新引入猪品种肉质的潜力。对新鲜肉样的质子 NMR T(2)弛豫曲线进行多元数据分析,揭示了研究中包含的不同品种之间 T(2)弛豫模式的差异。将 NMR T(2)弛豫数据与水保持能力(WHC)的重量测定进行比较表明,这应归因于不同肉类 WHC 的差异,并且根据先前的研究,NMR T(2)弛豫提供了有关 WHC 的独特信息,这可能归因于 NMR T(2)弛豫反映了有关内在肉质结构的信息。不同品种之间的肉代谢物图谱也通过冷冻渗出物和肉提取物的高分辨率质子 NMR 波谱进行了比较。还观察到了品种对冷冻渗出物中乳酸含量的影响,这可能与肉的 WHC 有关。总之,所应用的不同 NMR 技术可以提供有关生物物理和生物化学因素的互补信息,这些因素对于肉质评估很重要。