用于通过GM1酶联免疫吸附测定法简单检测大肠杆菌热稳定肠毒素的重组融合蛋白。
Recombinant fusion protein for simple detection of Escherichia coli heat-stable enterotoxin by GM1 enzyme-linked immunosorbent assay.
作者信息
Sanchez J, Holmgren J, Svennerholm A M
机构信息
Center for Research on Infectious Diseases, National Public Health Institute, Cuernavaca, Morelos, Mexico.
出版信息
J Clin Microbiol. 1990 Oct;28(10):2175-7. doi: 10.1128/jcm.28.10.2175-2177.1990.
Abstract
A recombinant gene fusion protein composed of an Escherichia coli heat-stable enterotoxin (STa) peptide epitope fused to the amino end of the cholera toxin B subunit was used to detect STa produced by clinical isolates of enterotoxigenic E. coli (STa-ETEC) by a single monoclonal antibody-based inhibition GM1 enzyme-linked immunosorbent assay. In this test, 100% sensitivity and 100% specificity were observed for use of the recombinant protein in either its purified form or as crude Vibrio cholerae culture supernatants in detection of STa-ETEC.
摘要
一种重组基因融合蛋白，由融合至霍乱毒素B亚基氨基端的大肠杆菌热稳定肠毒素（STa）肽表位组成，用于通过基于单克隆抗体的抑制GM1酶联免疫吸附测定法检测产肠毒素大肠杆菌（STa-ETEC）临床分离株产生的STa。在该试验中，观察到该重组蛋白以纯化形式或作为霍乱弧菌粗培养上清液用于检测STa-ETEC时，灵敏度和特异性均为100%。
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Development of a radioimmunoassay for Escherichia coli heat-stable enterotoxin: comparison with the suckling mouse bioassay.一种用于检测大肠杆菌热稳定肠毒素的放射免疫测定法的开发：与乳鼠生物测定法的比较。
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Recombinant system for overexpression of cholera toxin B subunit in Vibrio cholerae as a basis for vaccine development.用于霍乱弧菌中霍乱毒素B亚基过表达的重组系统作为疫苗开发的基础。
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