Cryan B
Department of Medical Microbiology, Regional Hospital, Galway, Republic of Ireland.
J Infect. 1989 Jan;18(1):59-66. doi: 10.1016/s0163-4453(89)93692-x.
Enterotoxigenic Escherichia coli (ETEC) are well recognised enteric pathogens. The epidemiology of heat-stable enterotoxin (STa) producing strains has not been established due mainly to difficulties encountered in performing bioassays on a large scale in baby mice. This study describes a competitive enzyme-linked immunosorbent assay (ELISA) for detecting STa and its application to clinical isolates. Compared to the bioassay, the ELISA had a specificity of 93.7% and a sensitivity of 90.9%. It detected as little as 1 microgram/l STa. Of 720 E. coli isolates from children with diarrhoea, 69 (9.6%) were positive for STa by ELISA.
产肠毒素大肠杆菌（ETEC）是公认的肠道病原体。主要由于在对幼鼠进行大规模生物测定时遇到困难，产热稳定肠毒素（STa）菌株的流行病学尚未确立。本研究描述了一种用于检测STa的竞争性酶联免疫吸附测定（ELISA）及其在临床分离株中的应用。与生物测定相比，ELISA的特异性为93.7%，灵敏度为90.9%。它能检测低至1微克/升的STa。在从腹泻儿童中分离出的720株大肠杆菌中，有69株（9.6%）通过ELISA检测STa呈阳性。
