一种用于检测大肠杆菌热稳定肠毒素的竞争性酶联免疫吸附测定法及其在临床分离株中的应用。

A competitive enzyme-linked immunosorbent assay for detecting Escherichia coli heat-stable enterotoxin and its application to clinical isolates.

作者信息

Cryan B

机构信息

Department of Medical Microbiology, Regional Hospital, Galway, Republic of Ireland.

出版信息

J Infect. 1989 Jan;18(1):59-66. doi: 10.1016/s0163-4453(89)93692-x.

DOI:10.1016/s0163-4453(89)93692-x

PMID:2644360

Abstract

Enterotoxigenic Escherichia coli (ETEC) are well recognised enteric pathogens. The epidemiology of heat-stable enterotoxin (STa) producing strains has not been established due mainly to difficulties encountered in performing bioassays on a large scale in baby mice. This study describes a competitive enzyme-linked immunosorbent assay (ELISA) for detecting STa and its application to clinical isolates. Compared to the bioassay, the ELISA had a specificity of 93.7% and a sensitivity of 90.9%. It detected as little as 1 microgram/l STa. Of 720 E. coli isolates from children with diarrhoea, 69 (9.6%) were positive for STa by ELISA.

摘要

产肠毒素大肠杆菌（ETEC）是公认的肠道病原体。主要由于在对幼鼠进行大规模生物测定时遇到困难，产热稳定肠毒素（STa）菌株的流行病学尚未确立。本研究描述了一种用于检测STa的竞争性酶联免疫吸附测定（ELISA）及其在临床分离株中的应用。与生物测定相比，ELISA的特异性为93.7%，灵敏度为90.9%。它能检测低至1微克/升的STa。在从腹泻儿童中分离出的720株大肠杆菌中，有69株（9.6%）通过ELISA检测STa呈阳性。