Wu Hai-Qiang, Li Li, Li Jing, He Zhen-Dan, Liu Zhi-Gang, Zeng Qing-Qian, Wang Yu-Sheng
College of Life Sciences, Shenzhen University, Shenzhen, China.
Chem Pharm Bull (Tokyo). 2012;60(2):178-82. doi: 10.1248/cpb.60.178.
This study characterized the acaricidal activity of constituents of patchouli oil extracted from (Pogostemon cablin (Blanco) BENTH) against the house dust mite, Dermatophagoides farinae. A new compound, 2-(1,3-dihydroxy-but-2-enylidene)-6-methyl-3-oxo-heptanoic acid (DHEMH), was isolated from patchouli oil and characterized by (1)H-NMR, (13)C-NMR, LC-MS and elemental analysis (EA). This active component was identified as the hydrolysate of pogostone. Fifteen other constituents found in patchouli oil were also identified by GC-MS, including patchouli alcohol and pogostone. LD(50) studies carried out over 24 h using contact toxicity tests identified DHEMH as the most toxic compound to D. farinae (2.04 μg/cm(2)), followed by patchouli oil (6.11 μg/cm(2)), benzyl benzoate (BP) (9.31 μg/cm(2)) and dibutyl phthalate (DBP) (58.52 μg/cm(2)). In vapor phase toxicity tests, all of these compounds were more effective in closed than open containers, indicating that the most efficient mode of delivery for these compounds is the vapor phase. These results indicate that DHEMH and patchouli oil merit further study as potential agents for the control of D. farinae.
本研究对从广藿香（Pogostemon cablin (Blanco) BENTH）中提取的广藿香油成分对屋尘螨（Dermatophagoides farinae）的杀螨活性进行了表征。从广藿香油中分离出一种新化合物，2-(1,3-二羟基-丁-2-亚烯基)-6-甲基-3-氧代-庚酸（DHEMH），并通过(1)H-NMR、(13)C-NMR、LC-MS和元素分析（EA）对其进行了表征。该活性成分被鉴定为百秋李醇的水解产物。广藿香油中发现的其他15种成分也通过GC-MS进行了鉴定，包括广藿香醇和百秋李醇。通过接触毒性试验进行的24小时LD(50)研究确定DHEMH是对粉尘螨毒性最大的化合物（2.04μg/cm(2)），其次是广藿香油（6.11μg/cm(2)）、苯甲酸苄酯（BP）（9.31μg/cm(2)）和邻苯二甲酸二丁酯（DBP）（58.52μg/cm(2)）。在气相毒性试验中，所有这些化合物在密闭容器中比在开放容器中更有效，这表明这些化合物最有效的递送方式是气相。这些结果表明，DHEMH和广藿香油作为控制粉尘螨的潜在药物值得进一步研究。
