通过聚合酶链反应鉴别编码大肠杆菌维罗毒素2和与猪水肿病相关的维罗毒素（VTe）的基因。
Differentiation of genes coding for Escherichia coli verotoxin 2 and the verotoxin associated with porcine edema disease (VTe) by the polymerase chain reaction.
作者信息
Johnson W M, Pollard D R, Lior H, Tyler S D, Rozee K R
机构信息
National Laboratory for Enteric Pathogens, Laboratory Centre for Disease Control, Ottawa, Ontario, Canada.
出版信息
J Clin Microbiol. 1990 Oct;28(10):2351-3. doi: 10.1128/jcm.28.10.2351-2353.1990.
Abstract
Two sets of synthetic oligonucleotide primers were used in a polymerase chain reaction adaptation to distinguish the closely related genes for type 2 verotoxin (VT2 or Shiga-like toxin [SLT-II]) and the verotoxin associated with porcine edema disease (VTe or SLT-II variant [SLT-IIv]) in Escherichia coli.
摘要
在聚合酶链反应中使用了两组合成寡核苷酸引物，以区分大肠杆菌中与2型志贺毒素（VT2或类志贺毒素[SLT-II]）密切相关的基因，以及与猪水肿病相关的志贺毒素（VTe或类志贺毒素-II变体[SLT-IIv]）。
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