Kobayashi Hideki, Kanazaki Mika, Hata Eiji, Kubo Masanori
National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
Appl Environ Microbiol. 2009 Jan;75(1):292-5. doi: 10.1128/AEM.01534-08. Epub 2008 Nov 7.
The prevalence and characteristics of eae- and stx-positive Escherichia coli strains in wild birds in the immediate environment of Tokyo Bay, Japan, was examined using cloacal swab samples taken from 447 birds belonging to 62 species. PCR screening showed that the prevalences of stx- and eae-positive strains of Escherichia coli were 5% (23/447) and 25% (113/447), respectively. Four strains of stx(2f)-positive E. coli were isolated from two feral pigeons, an oriental turtle dove and a barn swallow. In contrast, 39 eae-positive E. coli strains were isolated, and most of the strains possessed a subtype of intimin that is classified as a minor group of human intimins, such as intimin upsilon, kappa, and mu. Moreover, these strains did not possess any of the other pathogenic genes tested, such as stxs, ehxA, bfp, or irp. Thus, wild birds were considered to be a reservoir of atypical enteropathogenic E. coli.
利用从属于62个物种的447只鸟类采集的泄殖腔拭子样本，对日本东京湾周边野生鸟类中肠出血性大肠杆菌（eae）和志贺毒素（stx）阳性大肠杆菌菌株的流行情况及特征进行了检测。PCR筛查显示，大肠杆菌stx阳性菌株和eae阳性菌株的流行率分别为5%（23/447）和25%（113/447）。从两只野生鸽子、一只斑鸠和一只家燕中分离出4株stx（2f）阳性大肠杆菌。相比之下，分离出39株eae阳性大肠杆菌菌株，大多数菌株具有内膜蛋白的一个亚型，该亚型被归类为人类内膜蛋白的一个小群体，如内膜蛋白upsilon、kappa和mu。此外，这些菌株不具备所检测的任何其他致病基因，如stx、ehxA、bfp或irp。因此，野生鸟类被认为是非典型肠致病性大肠杆菌的储存宿主。
