用于鉴定星状诺卡菌的DNA探针。

DNA probes for the identification of Nocardia asteroides.

作者信息

Brownell G H, Belcher K E

机构信息

Department of Cell and Molecular Biology, Medical College of Georgia, Augusta 30912.

出版信息

J Clin Microbiol. 1990 Sep;28(9):2082-6. doi: 10.1128/jcm.28.9.2082-2086.1990.

DOI:10.1128/jcm.28.9.2082-2086.1990

PMID:2229389

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC268107/

Abstract

DNA probes for the rapid identification of Nocardia asteroides were obtained by constructing a genomic library of strain GUH-2 in the lambda cloning vector EMBL3. Of 50 recombinant clones tested, 2 were identified that hybridized with 31% of the N. asteroides strains in a reference collection without cross-hybridization with related members of the Actinomycetales. Additional libraries were then generated from selected strains of N. asteroides that had failed to hybridize with any of the GUH-2 clones. Four additional clones were obtained from these strains which, when pooled, provided DNA probes specific for all of the N. asteroides strains tested.

摘要

通过在λ克隆载体EMBL3中构建星状诺卡菌GUH-2菌株的基因组文库，获得了用于快速鉴定星状诺卡菌的DNA探针。在测试的50个重组克隆中，鉴定出2个与参考菌库中31%的星状诺卡菌菌株杂交，且不与放线菌目相关成员交叉杂交。然后从未能与任何GUH-2克隆杂交的星状诺卡菌选定菌株中构建了额外的文库。从这些菌株中又获得了4个克隆，将它们合并后可提供针对所有测试的星状诺卡菌菌株的DNA探针。