Edjème-Aké Angèle, Garnotel Roselyne, Vallé-Polneau Sandrine, Ahiboh Hugues, Hauhouot-Attoungbré Marie Laure, Monnet Dagui, Gillery Philippe
Laboratoire de biochimie, UFR sciences pharmaceutiques et biologiques, Université de Cocody, Abidjan, Côte d'Ivoire.
Ann Biol Clin (Paris). 2012 Jan-Feb;70(1):13-7. doi: 10.1684/abc.2011.0651.
This study reports the comparison between two methods (chemiluminescence and enzymatic colorimetry) for revelation of apolipoprotein(a) [apo(a)] isoforms by immunoblotting in 102 Ivorian healthy subjects. Apo(a) isoform sizes were determined by sodium dodecyl sulfate-agarose-polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunoblotting using enzymatic colorimetry or chemiluminescence. Within-run precision was comprised between 4.9% and 9.2% for colorimetry and between 2.9% and 4.6% for chemiluminescence. Both methods have detected apo(a) isoforms in all patients, even when lipoprotein(a) concentrations were under detection limit (0.02 g/L). The two methods were significantly correlated (r = 0.96 to 0.98, p<0.0001). Even though the chemiluminescence method exhibited better performances than the colorimetric method, both techniques could be used indifferently.
