• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

淀粉结合结构域的环状移位：配体选择性反转和亲和力增加。

Circular permutation of the starch-binding domain: inversion of ligand selectivity with increased affinity.

机构信息

Institute of Bioinformatics and Structural Biology, National Tsing Hua University, No. 101, Sec. 2, Kuang Fu Rd, Hsinchu, 30013, Taiwan ROC.

出版信息

Chem Commun (Camb). 2012 Mar 7;48(20):2612-4. doi: 10.1039/c2cc17376j. Epub 2012 Feb 1.

DOI:10.1039/c2cc17376j
PMID:22294161
Abstract

Proteins containing starch-binding domains (SBDs) are used in a variety of scientific and technological applications. A circularly permutated SBD (CP90) with improved affinity and selectivity toward longer-chain carbohydrates was synthesized, suggesting that a new starch-binding protein may be developed for specific scientific and industrial applications.

摘要

含有淀粉结合结构域（SBD）的蛋白质被应用于各种科学和技术应用中。合成了一种具有更高亲和力和选择性的环状排列淀粉结合结构域（CP90），对长链碳水化合物具有更高的亲和力和选择性，这表明可能开发出一种新的淀粉结合蛋白，用于特定的科学和工业应用。

相似文献

1
Circular permutation of the starch-binding domain: inversion of ligand selectivity with increased affinity.淀粉结合结构域的环状移位：配体选择性反转和亲和力增加。
Chem Commun (Camb). 2012 Mar 7;48(20):2612-4. doi: 10.1039/c2cc17376j. Epub 2012 Feb 1.
2
Role of the N-terminal starch-binding domains in the kinetic properties of starch synthase III from Arabidopsis thaliana.拟南芥淀粉合酶III的N端淀粉结合结构域在动力学特性中的作用
Biochemistry. 2008 Mar 4;47(9):3026-32. doi: 10.1021/bi702418h. Epub 2008 Feb 9.
3
Crystal structure of circular permuted RoCBM21 (CP90): dimerisation and proximity of binding sites.环形排列的 RoCBM21（CP90）的晶体结构：二聚化和结合位点的接近。
PLoS One. 2012;7(11):e50488. doi: 10.1371/journal.pone.0050488. Epub 2012 Nov 30.
4
Both binding sites of the starch-binding domain of Aspergillus niger glucoamylase are essential for inducing a conformational change in amylose.黑曲霉葡萄糖淀粉酶淀粉结合结构域的两个结合位点对于诱导直链淀粉的构象变化都是必不可少的。
J Mol Biol. 2001 Nov 9;313(5):1149-59. doi: 10.1006/jmbi.2001.5097.
5
Thermodynamics of binding of heterobidentate ligands consisting of spacer-connected acarbose and beta-cyclodextrin to the catalytic and starch-binding domains of glucoamylase from Aspergillus niger shows that the catalytic and starch-binding sites are in close proximity in space.由间隔连接的阿卡波糖和β-环糊精组成的异双齿配体与黑曲霉葡糖淀粉酶的催化结构域和淀粉结合结构域结合的热力学表明，催化位点和淀粉结合位点在空间上紧密相邻。
Biochemistry. 1998 Jul 21;37(29):10446-52. doi: 10.1021/bi9807310.
6
Glucoamylase starch-binding domain of Aspergillus niger B1: molecular cloning and functional characterization.黑曲霉B1的葡糖淀粉酶淀粉结合结构域：分子克隆与功能表征
Biochem J. 2003 Jun 15;372(Pt 3):905-10. doi: 10.1042/BJ20021527.
7
The carbohydrate-binding module family 20--diversity, structure, and function.碳水化合物结合模块家族20——多样性、结构与功能
FEBS J. 2009 Sep;276(18):5006-29. doi: 10.1111/j.1742-4658.2009.07221.x. Epub 2009 Aug 13.
8
Improving the amylolytic activity of Saccharomyces cerevisiae glucoamylase by the addition of a starch binding domain.通过添加淀粉结合结构域提高酿酒酵母葡萄糖淀粉酶的淀粉分解活性。
J Biotechnol. 2005 Aug 4;118(2):167-76. doi: 10.1016/j.jbiotec.2005.03.019.
9
Alpha-amylase starch binding domains: cooperative effects of binding to starch granules of multiple tandemly arranged domains.α-淀粉酶淀粉结合结构域：多个串联排列结构域与淀粉颗粒结合的协同效应。
Appl Environ Microbiol. 2007 Jun;73(12):3833-7. doi: 10.1128/AEM.02628-06. Epub 2007 Apr 27.
10
Crystal structure of the polyextremophilic alpha-amylase AmyB from Halothermothrix orenii: details of a productive enzyme-substrate complex and an N domain with a role in binding raw starch.嗜盐嗜热栖热放线菌多极端嗜热α-淀粉酶AmyB的晶体结构：活性酶-底物复合物及在结合生淀粉中起作用的N结构域的细节
J Mol Biol. 2008 May 9;378(4):852-70. doi: 10.1016/j.jmb.2008.02.041. Epub 2008 Feb 29.

引用本文的文献

1
Resources and Methods for Engineering "Designer" Glycan-Binding Proteins.工程“设计”糖结合蛋白的资源和方法。
Molecules. 2021 Jan 13;26(2):380. doi: 10.3390/molecules26020380.
2
Functional demonstrations of starch binding domains present in Ostreococcus tauri starch synthases isoforms.莱茵衣藻淀粉合酶同工型中存在的淀粉结合结构域的功能验证。
BMC Res Notes. 2015 Oct 28;8:613. doi: 10.1186/s13104-015-1598-6.
3
Crystal structure of circular permuted RoCBM21 (CP90): dimerisation and proximity of binding sites.环形排列的 RoCBM21（CP90）的晶体结构：二聚化和结合位点的接近。
PLoS One. 2012;7(11):e50488. doi: 10.1371/journal.pone.0050488. Epub 2012 Nov 30.
4
Circular permutation prediction reveals a viable backbone disconnection for split proteins: an approach in identifying a new functional split intein.环状重排预测揭示了可分裂蛋白质的可行骨架断开：一种识别新功能分裂内含肽的方法。
PLoS One. 2012;7(8):e43820. doi: 10.1371/journal.pone.0043820. Epub 2012 Aug 24.