Department of Biology, Centre for Applied Synthetic Biology, and Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, QC H4B 1R6, Canada.
PROTEO, Quebec Network for Research on Protein Function, Structure, and Engineering, Quebec City, QC G1V 0A6, Canada.
Molecules. 2021 Jan 13;26(2):380. doi: 10.3390/molecules26020380.
This review provides information on available methods for engineering glycan-binding proteins (GBP). Glycans are involved in a variety of physiological functions and are found in all domains of life and viruses. Due to their wide range of functions, GBPs have been developed with diagnostic, therapeutic, and biotechnological applications. The development of GBPs has traditionally been hindered by a lack of available glycan targets and sensitive and selective protein scaffolds; however, recent advances in glycobiology have largely overcome these challenges. Here we provide information on how to approach the design of novel "designer" GBPs, starting from the protein scaffold to the mutagenesis methods, selection, and characterization of the GBPs.
这篇综述提供了关于糖基结合蛋白（GBP）工程化的可用方法的信息。聚糖参与多种生理功能，存在于所有生命领域和病毒中。由于其广泛的功能，GBP 已被开发用于诊断、治疗和生物技术应用。GBP 的开发传统上受到可用聚糖靶标和敏感选择性蛋白质支架的限制；然而，糖生物学的最新进展在很大程度上克服了这些挑战。在这里，我们提供了如何从蛋白质支架开始设计新型“设计”GBP 的信息，包括突变方法、GBP 的选择和表征。
