Renninger Jonathan P
GlaxoSmithKline Pharmaceuticals, King of Prussia, Pennsylvania, USA.
Curr Protoc Pharmacol. 2006 Jul;Chapter 10:Unit10.11. doi: 10.1002/0471141755.ph1011s33.
The importance of the ability to accurately evaluate respiratory function in animals has been underscored by the classification of the respiratory system as a vital organ system by regulatory agencies in the United States, the European Union, and Japan. A comprehensive assessment of respiratory function should include an evaluation of ventilatory function, including rate and volume measurements, overall pulmonary ventilation (i.e., minute volume), and lung function, including resistance to lung airflow. A volume-displacement, head-out, plethysmograph chamber is widely considered the most accurate method for measuring respiratory flows and volumes in small animals because it allows direct assessment of both volume and rate as well as resistance to lung airflow, a predictor of airway constriction or obstruction. Direct assessment of airway resistance is important because mild to moderate increases are generally not detected as changes in ventilatory patterns. This unit describes a method for evaluating ventilatory and lung function in conscious rats.
美国、欧盟和日本的监管机构将呼吸系统列为重要器官系统，这凸显了准确评估动物呼吸功能能力的重要性。对呼吸功能的全面评估应包括对通气功能的评估，包括速率和容量测量、整体肺通气（即分钟通气量），以及肺功能评估，包括肺气流阻力。容积置换式、头部外置的体积描记箱被广泛认为是测量小动物呼吸流量和容积的最准确方法，因为它可以直接评估容积、速率以及肺气流阻力，而肺气流阻力是气道收缩或阻塞的一个预测指标。直接评估气道阻力很重要，因为轻度至中度的增加通常不会表现为通气模式的变化。本单元介绍了一种评估清醒大鼠通气和肺功能的方法。
