van Dam P A, Watson J V, Lowe D G, Cox H, Curling M, Shepherd J H
Department of Obstetrics and Gynaecology, St Bartholomew's Hospital, West Smithfield, London.
J Clin Pathol. 1990 Oct;43(10):833-9. doi: 10.1136/jcp.43.10.833.
A multiparameter flow cytometric assay for the simultaneous study of tumour associated antigens (TAA) and DNA in fresh solid tumours was devised. Cell suspensions were prepared by disaggregating unfixed solid tumour samples mechanically over a stainless steel mesh. Indirect immunofluorescence was used to identify the TAA, and DNA was stained with propidium iodide. Cell morphology was well preserved, cell clumping was negligible, and high quality indirect immunofluorescence quality indirect immunofluorescence and DNA staining were obtained. The technique is simple, rapid, and reproducible. Multiparameter assays can be developed to study prognostic indicators such as membrane oncoproteins, receptors, and multidrug resistance in solid tumours. With a suitable panel of antibodies the technique might become an aid in the differential diagnosis and biochemical diagnosis of some solid tumours.
设计了一种多参数流式细胞术检测方法，用于同时研究新鲜实体瘤中的肿瘤相关抗原（TAA）和DNA。通过在不锈钢筛网上机械解离未固定的实体瘤样本制备细胞悬液。采用间接免疫荧光法鉴定TAA，并用碘化丙啶对DNA进行染色。细胞形态得到良好保存，细胞聚集可忽略不计，获得了高质量的间接免疫荧光和DNA染色结果。该技术简单、快速且可重复。可以开发多参数检测方法来研究实体瘤中的预后指标，如膜癌蛋白、受体和多药耐药性。使用合适的抗体组合，该技术可能有助于某些实体瘤的鉴别诊断和生化诊断。
