Oertel J, Oertel B, Lobeck H, Huhn D
Haematologische Abteilung, Freie Universitat Berlin, West Germany.
J Clin Pathol. 1990 Oct;43(10):844-6. doi: 10.1136/jcp.43.10.844.
Thirty four patients positive for human immunodeficiency virus (HIV) who had lymphadenopathy were investigated using fine needle aspiration. Cytological analysis included immunocytochemical investigation with the alkaline phosphatase-antialkaline phosphatase (APAAP) method. All patients had confirmation of cytological diagnosis by lymph node biopsy. Fifteen aspirates with follicular hyperplasia were evaluated. Eleven patients showed B cell predominance. The B cell population did not show light chain restriction. Ten patients with B cell non-Hodgkin's lymphoma (five with Burkitt's lymphoma and five with B cell immunoblastic lymphoma) were investigated. Nine out of 10 cases were monoclonal with respect to their light chain determinants; only one case with Burkitt's lymphoma with partial lymph node metastasis did not show light chain restriction. The cytological diagnosis included two mycobacterial infections and four cystic lesions. Histological investigation was necessary to diagnose the extent of lymph node disease caused by Kaposi's sarcoma. These findings indicate that the immunocytological investigation of lymph node aspirates is useful for evaluating lymphadenopathy in HIV positive patients.
对34例人类免疫缺陷病毒（HIV）阳性且有淋巴结病的患者进行了细针穿刺抽吸检查。细胞学分析包括采用碱性磷酸酶 - 抗碱性磷酸酶（APAAP）法进行免疫细胞化学研究。所有患者均通过淋巴结活检证实了细胞学诊断。对15例有滤泡增生的抽吸物进行了评估。11例患者显示B细胞占优势。B细胞群体未显示轻链限制。对10例B细胞非霍奇金淋巴瘤患者（5例伯基特淋巴瘤和5例B细胞免疫母细胞淋巴瘤）进行了研究。10例中有9例在轻链决定簇方面是单克隆的；只有1例有部分淋巴结转移的伯基特淋巴瘤未显示轻链限制。细胞学诊断包括2例分枝杆菌感染和4例囊性病变。对于诊断卡波西肉瘤引起的淋巴结疾病范围，组织学检查是必要的。这些发现表明，对淋巴结抽吸物进行免疫细胞学研究有助于评估HIV阳性患者的淋巴结病。
