Erber W N, Pinching A J, Mason D Y
Lancet. 1984 May 12;1(8385):1042-6. doi: 10.1016/s0140-6736(84)91451-x.
Immunofluorescence labelling of peripheral blood mononuclear cells is usually done on cells in suspension. This paper describes a procedure based on immuno-alkaline phosphatase staining of routine blood smears. The advantages of this method are that a few drops of blood are sufficient for labelling multiple lymphocyte subpopulations; smears may be stored for long periods before labelling; it is unnecessary to isolate a mononuclear cell fraction before labelling; labelled preparations can be stored; and the morphological features of labelled cells are shown clearly. The technique was used to label T cells and their subsets, B cells, and HLA-DR antigen in blood smears from 15 normal donors, from 7 patients with infectious mononucleosis, from 1 patient with clinically proven AIDS, and from 1 symptom-free subject at risk of AIDS. The normal T helper/suppressor ratio of 1 X 95 was reversed in all of the last three groups of subjects, the mean being 0 X 34 for infectious mononucleosis; the value was 0 X 22 in the AIDS patients. Immuno-alkaline phosphatase labelling of routine blood smears seems to be a valuable method for studying abnormalities in circulating lymphocyte subpopulations and lends itself to mass screening for altered T helper and T suppressor subjects-for example, in blood donors.
外周血单个核细胞的免疫荧光标记通常是在悬浮细胞上进行的。本文描述了一种基于常规血涂片免疫碱性磷酸酶染色的方法。该方法的优点是几滴血液就足以标记多个淋巴细胞亚群；涂片在标记前可长期保存；标记前无需分离单个核细胞部分；标记后的制剂可保存；并且标记细胞的形态特征清晰可见。该技术用于标记15名正常供者、7名传染性单核细胞增多症患者、1名临床确诊的艾滋病患者以及1名有艾滋病风险的无症状受试者血涂片中的T细胞及其亚群、B细胞和HLA-DR抗原。后三组受试者的正常T辅助/抑制细胞比值1×95均发生了逆转，传染性单核细胞增多症患者的平均值为0×34；艾滋病患者的值为0×22。常规血涂片的免疫碱性磷酸酶标记似乎是研究循环淋巴细胞亚群异常的一种有价值的方法，适用于对T辅助细胞和T抑制细胞改变的受试者进行大规模筛查，例如在献血者中。
