Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Japan.
Biotechnol J. 2012 May;7(5):686-9. doi: 10.1002/biot.201100432. Epub 2012 Feb 29.
Glutathione is a valuable tripeptide that is widely used in the pharmaceutical, food, and cosmetic industries. Glutathione is industrially produced by fermentation using Saccharomyces cerevisiae. Before the glutathione fermentation process with S. cerevisiae, a glucose extraction process from starchy materials is required. This glucose extraction is usually carried out by converting starchy materials to starch using high-temperature cooking and subsequent hydrolysis by amylases to convert starch to glucose. In this study, to develop an energy-saving glutathione production process by reducing energy consumption during the cooking step, we efficiently produced glutathione from low-temperature cooked rice using amylase-expressing S. cerevisiae. The combination of the amylase-expressing yeast with low-temperature cooking is potentially applicable to a variety of energy-saving bio-production methods of chemicals from starchy bio-resources.
谷胱甘肽是一种有价值的三肽，广泛应用于医药、食品和化妆品行业。谷胱甘肽通过使用酿酒酵母进行发酵在工业上生产。在使用酿酒酵母进行谷胱甘肽发酵之前，需要从淀粉质材料中提取葡萄糖。这种葡萄糖提取通常通过使用高温蒸煮将淀粉质材料转化为淀粉，然后使用淀粉酶将淀粉水解为葡萄糖来完成。在这项研究中，为了通过减少蒸煮步骤的能耗来开发节能的谷胱甘肽生产工艺，我们使用表达淀粉酶的酿酒酵母从低温蒸煮的米饭中高效生产谷胱甘肽。表达淀粉酶的酵母与低温蒸煮的结合可能适用于从淀粉质生物资源中节约能源的各种化学品的生物生产方法。
