Katsura Research Laboratory Bio and Medical Products Research Department, Sanyo Chemical Industries Ltd, Kyoto, Japan.
J Tissue Eng Regen Med. 2013 May;7(5):348-52. doi: 10.1002/term.527. Epub 2012 Jan 31.
This paper is one trial of surface modification of titanium with pronectin F+ (PN) of an artificial protein to enhance gingival adhesion. Titanium plates were electrodeposited in the PN solution to prepare PN-electrodeposited titanium plates. When PN detachment from the PN-electrodeposited titanium plates was investigated, no detachment was observed, in contrast to the case of titanium plates simply coated with PN. A cell culture experiment demonstrated that electrodeposited PN had an inherent ability to enhance the initial attachment of gingival epithelial cells. The PN-electrodeposited titanium plates were implanted between the gingival epithelium and the underlying bone tissue of rabbits to evaluate epithelial growth on the plates and their gingival adhesion. Non-treated and PN-coated titanium plates were used as controls. PN electrodeposition enhanced epithelial growth and adhesion of titanium plates to a significantly great extent compared with PN-coated plates. These findings demonstrate that PN electrodeposition is a promising method to enhance epithelium adhesion onto a titanium surface.
本文是通过将人工蛋白 pronectin F+（PN）接枝到钛表面，从而增强牙龈黏附力，来对钛进行表面改性的尝试。通过将钛板电沉积到 PN 溶液中，制备出 PN 电沉积钛板。当研究 PN 从 PN 电沉积钛板上的脱落情况时，与简单涂覆 PN 的钛板相比，未观察到脱落。细胞培养实验表明，电沉积 PN 具有增强牙龈上皮细胞初始黏附的固有能力。将 PN 电沉积钛板植入兔的牙龈上皮和下方骨组织之间，以评估上皮细胞在钛板上的生长及其与牙龈的黏附情况。未处理和涂覆 PN 的钛板被用作对照。与涂覆 PN 的钛板相比，PN 电沉积显著增强了上皮细胞的生长和钛板的牙龈黏附。这些发现表明，PN 电沉积是增强钛表面上皮黏附的一种很有前途的方法。
