Jaing Crystal, Gardner Shea, McLoughlin Kevin, Thissen James B, Slezak Tom
Physical & Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA.
PDA J Pharm Sci Technol. 2011 Nov-Dec;65(6):668-74. doi: 10.5731/pdajpst.2011.00838.
CONFERENCE PROCEEDING Proceedings of the PDA/FDA Adventitious Viruses in Biologics: Detection and Mitigation Strategies Workshop in Bethesda, MD, USA; December 1-3, 2010 Guest Editors: Arifa Khan (Bethesda, MD), Patricia Hughes (Bethesda, MD) and Michael Wiebe (San Francisco, CA) We designed the Lawrence Livermore Microbial Detection Array (LLMDA), which contains 388,000 DNA probes. This array can detect any sequenced viruses or bacteria within 24 h. In addition, the oligonucleotide probes were selected to enable detection of novel, divergent species with homology to sequenced organisms. We recently used this array to identify an adventitious virus from a vaccine product. We have also used this array to detect viral and bacterial infections from various human clinical samples. Broad-spectrum microbial detection microarrays are efficient and cost-effective tools to rapidly screen cell bank samples, raw materials, vaccine samples, and clinical samples to ensure drug, food, and health safety in the United States and worldwide.
会议论文集 《PDA/FDA生物制品中潜在病毒：检测与缓解策略研讨会论文集》，美国马里兰州贝塞斯达；2010年12月1日至3日 特邀编辑：阿里法·汗（马里兰州贝塞斯达）、帕特里夏·休斯（马里兰州贝塞斯达）和迈克尔·维贝（加利福尼亚州旧金山） 我们设计了劳伦斯利弗莫尔微生物检测阵列（LLMDA），它包含388,000个DNA探针。该阵列能够在24小时内检测出任何已测序的病毒或细菌。此外，所选的寡核苷酸探针能够检测出与已测序生物具有同源性的新型、不同种类的病毒。我们最近使用该阵列从一种疫苗产品中鉴定出一种潜在病毒。我们还使用该阵列检测来自各种人类临床样本的病毒和细菌感染。广谱微生物检测微阵列是高效且具成本效益的工具，可快速筛选细胞库样本、原材料、疫苗样本和临床样本，以确保美国及全球的药品、食品和健康安全。
