Chandler J E, Tirado V, Adkinson R W
Louisiana State University Agricultural Center, Baton Rouge 70803.
J Dairy Sci. 1990 Aug;73(8):2129-35. doi: 10.3168/jds.S0022-0302(90)78893-5.
Chromosomal areas from metaphase spreads of male bovine leukocytes were digitized and sex chromosomes identified using videomicroscopy. Autosomal areas were ranked in descending order within a cell and assigned to two categories based on alternating rank. X and Y chromosome areas were assigned to respective categories. Areas were divided by 4 to make their sum equivalent to sperm DNA content. Data were analyzed before and after inclusion of sex chromosomal areas. Before X and Y inclusion, rank contributed to difference in chromosomal areas. Rank by category interaction and category effects did not contribute to area variation. After X and Y inclusion, area variation was due to rank by category interaction, rank, and category. Differences between sums of chromosomal areas across categories was 3.57%. Head areas of morphologically normal sperm with intact acrosomes were digitized using the same optics as chromosomal areas. Sum of corrected chromosomal areas per category was used in discriminant analysis to assign sperm head areas to two categories with .5 prior probabilities. Assignment resulted in 1037 sperm in one category and 1177 in the other. Difference between largest sperm head area classes across categories was 3.2%. Discrimination of sperm head areas, based on sum of chromosomal area and measured with computerized videomicroscopy, may be used to evaluate sex of bovine spermatozoa.
利用视频显微镜对雄性牛白细胞中期分裂相的染色体区域进行数字化处理，并识别性染色体。在一个细胞内，常染色体区域按降序排列，并根据交替排列分为两类。X和Y染色体区域分别归入相应类别。各区域除以4，使其总和等同于精子DNA含量。在纳入性染色体区域前后对数据进行分析。在纳入X和Y染色体之前，排列顺序导致染色体区域存在差异。类别交互作用和类别的排列顺序对区域变异没有影响。纳入X和Y染色体之后，区域变异是由于类别交互作用、排列顺序和类别造成的。不同类别染色体区域总和之间的差异为3.57%。使用与染色体区域相同的光学设备对顶体完整的形态正常精子的头部区域进行数字化处理。在判别分析中，使用每类校正后的染色体区域总和将精子头部区域分为两类，先验概率为0.5。分类结果是一类有1037个精子，另一类有1177个精子。不同类别中最大精子头部区域类别的差异为3.2%。基于染色体区域总和并通过计算机化视频显微镜测量对精子头部区域进行判别，可用于评估牛精子的性别。
